Cystatin S/CST4 蛋白, Human (HEK293, His)

Cystatin S (CST4) protein strongly inhibits papain and ficin, showing potent inhibition. Although it partially inhibits stem bromelain and bovine cathepsin C, it does not block the activity of porcine cathepsin B or clostridial protease. Cystatin S/CST4 Protein, Human (HEK293, His) is the recombinant human-derived Cystatin S/CST4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cystatin S/CST4 Protein, Human (HEK293, His) is 121 a.a., with molecular weight of ~16.0 kDa.

胱抑素 S (CST4) 蛋白对木瓜蛋白酶和无花果蛋白酶具有强大的抑制活性，表现出强大的抑制作用。此外，它还能部分抑制茎菠萝蛋白酶和牛组织蛋白酶 C，但不会阻碍猪组织蛋白酶 B 或梭菌蛋白酶的活性。值得注意的是，木瓜蛋白酶的抑制遵循非竞争性模式。这种多样化的抑制谱凸显了 CST4 在调节各种蛋白酶活性方面的特异性，表明其在调节蛋白水解过程中的作用。CST4 针对特定酶的独特抑制特性有助于其在细胞和生理环境中的功能意义，强调其对蛋白酶介导的途径和细胞稳态的潜在影响。

Human

HEK293

C-6*His

P01036 (S21-A141)

1472  [NCBI]

SSSKEENRIIPGGIYDADLNDEWVQRALHFAISEYNKATEDEYYRRPLQVLRAREQTFGGVNYFFDVEVGRTICTKSQPNLDTCAFHEQPELQKKQLCSFEIYEVPWEDRMSLVNSRCQEA

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 6% Trehalose, 4% Mannitol, 50mM NaCl, 0.05% Tween80, pH 9.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.