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  4. Cytoglobin/Histoglobin 蛋白, Human (His)

Cytoglobin/Histoglobin 蛋白, Human (His)

目录号: HY-P70089
产品使用指南

细胞珠蛋白/组织珠蛋白在保护细胞免受氧化应激方面发挥着至关重要的作用，可能充当细胞损伤的保护措施。此外，它还参与与氧储存或转移相关的细胞内过程。该蛋白质的结构完整性涉及通过二硫键连接的同源二聚体的形成，强调了其在细胞环境中分子相互作用和稳定性的能力。Cytoglobin/Histoglobin 蛋白, Human (His) 是重组的 细胞珠蛋白/组织珠蛋白，由 E. coli 表达，带有 C-6*His 标签。Cytoglobin/Histoglobin 蛋白, Human (His) 全长 190 个氨基酸，分子量约为 ~22.0 kDa。

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描述

Cytoglobin/Histoglobin Protein plays a vital role in protecting cells from oxidative stress, potentially acting as a safeguard against cellular damage. Additionally, it participates in intracellular processes related to oxygen storage or transfer. The protein's structural integrity involves the formation of homodimers linked by disulfide linkages, emphasizing its capacity for molecular interactions and stability within the cellular environment. Cytoglobin/Histoglobin Protein, Human (His) is the recombinant human-derived Cytoglobin/Histoglobin protein, expressed by E. coli , with C-6*His labeled tag. The total length of Cytoglobin/Histoglobin Protein, Human (His) is 190 a.a., with molecular weight of ~22.0 kDa.
研究背景

细胞珠蛋白/组织珠蛋白似乎在氧化应激期间保护细胞方面发挥着至关重要的作用，可能作为防止细胞损伤的保护机制。此外，它还涉及与氧储存或转移相关的细胞内过程。在结构上，该蛋白质形成通过二硫键互连的同二聚体，突出了其促进分子相互作用和维持细胞环境内稳定性的能力。
种属

Human
表达系统

E. coli
标签

C-6*His
蛋白编号

Q8WWM9 (M1-P190)
基因 ID
蛋白结构
N-term
CYGB (M1-P190)
Accession # Q8WWM9
6*His
C-term
同用名
rHuCytoglobin/Histoglobin, His; Cytoglobin; Histoglobin; HGb; Stellate Cell Activation-Associated Protein; CYGB; STAP
氨基酸序列

MEKVPGEMEIERRERSEELSEAERKAVQAMWARLYANCEDVGVAILVRFFVNFPSAKQYFSQFKHMEDPLEMERSPQLRKHACRVMGALNTVVENLHDPDKVSSVLALVGKAHALKHKVEPVYFKILSGVILEVVAEEFASDFPPETQRAWAKLRGLIYSHVTAAYKEVGWVQQVPNATTPPATLPSSGP

分子量

Approximately 22.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Cytoglobin/Histoglobin Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号：
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