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  3. D-hydantoinase 蛋白, Arthrobacter crystallopoietes (His)

D-hydantoinase 蛋白, Arthrobacter crystallopoietes (His)

目录号: HY-P702135
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D-乙内酰脲酶表现出独特的氨甲酰化谱,其中单个赖氨酸残基配位两个二价金属阳离子。该蛋白质属于金属依赖性水解酶超家族,特别是乙内酰脲酶/二氢嘧啶酶家族。D-hydantoinase 蛋白, Arthrobacter crystallopoietes (His) 是重组的 D-乙内酰脲酶蛋白,由 E. coli 表达,带有 N-6*His 标签。D-hydantoinase 蛋白, Arthrobacter crystallopoietes (His) 全长 457 个氨基酸。

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描述

D-hydantoinase exhibits a unique carbamylation profile in which a single lysine residue coordinates two divalent metal cations. This protein belongs to the metal-dependent hydrolase superfamily, specifically to the hydantoinase/dihydropyrimidase family. D-hydantoinase Protein, Arthrobacter crystallopoietes (His) is the recombinant D-hydantoinase protein, expressed by E. coli , with N-6*His labeled tag. The total length of D-hydantoinase Protein, Arthrobacter crystallopoietes (His) is 457 a.a., .

研究背景

D-乙内酰脲酶是一种属于金属依赖性水解酶超家族和乙内酰脲酶/二氢嘧啶酶家族的蛋白质,在氨甲酰化(单个赖氨酸残基协调两个二价金属阳离子的过程)中发挥着至关重要的作用。该蛋白质参与乙内酰脲水解,参与乙内酰脲转化为α-氨基酸。双金属配位强调了其在催化这些反应中的重要性。作为金属依赖性水解酶超家族的一员,D-乙内酰脲酶可能与相关酶具有保守的结构和功能特征。协调两种金属阳离子的能力进一步表明该酶家族内底物结合和催化的独特机制。

种属

Others

表达系统

E. coli

标签

N-6*His

蛋白编号

A0A1H1C1X0 (M1-R457)

基因 ID

/

蛋白结构
N-term
6*His
D-hydantoinase (M1-R457)
Accession # A0A1H1C1X0
C-term
中文名
D-hydantoinase 蛋白
同用名
Dihydropyrimidinase
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

D-hydantoinase Protein, Arthrobacter crystallopoietes (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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D-hydantoinase Protein, Arthrobacter crystallopoietes (His)
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HY-P702135
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