DAG1 蛋白, Human (HEK293, His)

DAG1 Proteinas, a pivotal extracellular glycoprotein, is crucial for laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Serving as a receptor for laminin-G domain-containing extracellular matrix proteins, DAG1 interacts with laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells and acts as a receptor for laminin LAMA5. DAG1 Protein, Human (HEK293, His) is the recombinant human-derived DAG1 protein, expressed by HEK293 , with C-His labeled tag. The total length of DAG1 Protein, Human (HEK293, His) is 720 a.a., with molecular weight (glycosylation form) of ~95-110 kDa, 55-95 kDa, 17-20 kDa.

肌营养不良聚糖 (DAG1) 在各种生物过程中发挥着至关重要的作用，参与层粘连蛋白和基底膜组装、肌膜稳定性、细胞存活、周围神经髓鞘形成、结节结构、细胞迁移和上皮极化。作为一种细胞外外周糖蛋白，DAG1 充当含有层粘连蛋白-G 结构域的细胞外基质蛋白的受体。具体而言，它在周围神经雪旺细胞中充当层粘连蛋白-2 (LAMA2) 和集聚蛋白的受体，并且还充当层粘连蛋白 LAMA5 的受体。

Human

HEK293

C-His

Q14118 (H30-V749)

1605  [NCBI]

HWPSEPSEAVRDWENQLEASMHSVLSDLHEAVPTVVGIPDGTAVVGRSFRVTIPTDLIASSGDIIKVSAAGKEALPSWLHWDSQSHTLEGLPLDTDKGVHYISVSATRLGANGSHIPQTSSVFSIEVYPEDHSELQSVRTASPDPGEVVSSACAADEPVTVLTVILDADLTKMTPKQRIDLLHRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIHGVEAPAREGAMSAQLGYPVVGWHIANKKPPLPKRVRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPGQIRPTMTIPGYVEPTAVATPPTTTTKKPRVSTPKPATPSTDSTTTTTRRPTKKPRTPRPVPRVTTKVSITRLETASPPTRIRTTTSGVPRGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDHEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDRAPARFKAKFVGDPALVLNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQIAGLSRRIAEDDGKPRPAFSNALEPDFKATSITVTGSGSCRHLQFIPVVPPRRVPSEAPPTEVPDRDPEKSSEDDV

Approximately 95-110 kDa, 55-95 kDa and 17-20 kDa due to glycosylation

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.