2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. DBI 蛋白, Mouse (P. pastoris, His)

DBI 蛋白, Mouse (P. pastoris, His)

目录号: HY-P700627
产品使用指南

DBI 蛋白具有高亲和力，可结合中链和长链酰基辅酶 A 酯，可能充当细胞内载体。此外，DBI 取代了 GABA A 型受体上苯二氮卓 (BZD) 识别位点的地西泮，表明神经肽在调节 GABA 受体作用中发挥着作用。从结构上看，作为一个单体，DBI 强调了其单个单元在细胞途径内不同分子相互作用中的重要性。DBI 蛋白, Mouse (P. pastoris, His) 是重组的 DBI 蛋白，由 P. pastoris 酵母表达，带有 N-6*His 标签。DBI 蛋白, Mouse (P. pastoris, His) 全长 86 个氨基酸，分子量为 11.9 kDa。

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描述

DBI Protein, with high affinity, binds medium- and long-chain acyl-CoA esters, potentially acting as an intracellular carrier. Moreover, DBI displaces diazepam from the benzodiazepine (BZD) recognition site on the GABA type A receptor, suggesting a neuropeptide role in modulating GABA receptor action. Structurally, as a monomer, DBI underscores the importance of its individual unit in diverse molecular interactions within cellular pathways. DBI Protein, Mouse (P. pastoris, His) is the recombinant mouse-derived DBI protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of DBI Protein, Mouse (P. pastoris, His) is 86 a.a., with molecular weight of 11.9 kDa.
研究背景

DBI 蛋白通过与中链和长链酰基辅酶 A 酯具有高亲和力结合，在细胞内过程中发挥着至关重要的作用，表明其作为这些分子的细胞内载体的潜在功能。此外，DBI 还具有从 GABA A 型受体上苯二氮卓 (BZD) 识别位点取代地西泮的能力。这种双重功能提出了 DBI 作为神经肽调节 GABA 受体作用的可能性。在结构上，该蛋白质作为单体存在，强调其单个单元在细胞途径内执行这些不同分子相互作用的重要性。
种属

Mouse
表达系统

P. pastoris
标签

N-6*His
蛋白编号

P31786 (S2-I87)
基因 ID
蛋白结构
N-term
6*His
DBI (S2-I87)
Accession # P31786
C-term
同用名
Dbi; diazepam binding inhibitor; EP; Acbp; ACBD1; endozepine; acyl-CoA-binding protein; diazepam binding inhibitor, splice form 1b
氨基酸序列

SQAEFDKAAEEVKRLKTQPTDEEMLFIYSHFKQATVGDVNTDRPGLLDLKGKAKWDSWNKLKGTSKESAMKTYVEKVDELKKKYGI

分子量

11.9 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DBI Protein, Mouse (P. pastoris, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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