2. 重组蛋白
  3. Ubiquitin Related Proteins
  4. DCAF16 蛋白, Human (Sf9, His)

DCAF16 蛋白, Human (Sf9, His)

目录号: HY-P701518
产品使用指南

DCAF16 蛋白是 CUL4-DDB1 E3 泛素-蛋白连接酶复合物中的重要组成部分，是关键的底物识别元件。它在核蛋白的泛素化和随后的蛋白酶体依赖性降解中发挥核心作用，强调其在蛋白质修饰过程中的关键参与，特别是在蛋白质泛素化的调节中。DCAF16 蛋白, Human (Sf9, His) 是重组的 DCAF16 蛋白，由 Sf9 insect cells 表达，带有 N-6*His 标签。DCAF16 蛋白, Human (Sf9, His) 全长 215 个氨基酸。

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描述

DCAF16 Protein, a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serves as a crucial substrate recognition element. It plays a central role in ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins, emphasizing its key involvement in protein modification processes, particularly in the regulation of protein ubiquitination. DCAF16 Protein, Human (Sf9, His) is the recombinant human-derived DCAF16 protein, expressed by Sf9 insect cells , with N-6*His labeled tag. The total length of DCAF16 Protein, Human (Sf9, His) is 215 a.a., .
研究背景

DCAF16 蛋白是 CUL4-DDB1 E3 泛素-蛋白连接酶复合物中的关键底物识别组件，在核蛋白的泛素化和随后的蛋白酶体依赖性降解中发挥核心作用。该功能强调了它参与蛋白质修饰过程，特别是蛋白质泛素化的调节。
种属

Human
表达系统

Sf9 insect cells
标签

N-6*His
蛋白编号

Q9NXF7 (G2-L216)
基因 ID

54876
蛋白结构
N-term
6*His
DCAF16 (G2-L216)
Accession # Q9NXF7
C-term
同用名
DCAF16; DDB1- and CUL4-associated factor 16
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

DCAF16 Protein, Human (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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