DCUN1D2 蛋白, Human (His)

The DCUN1D2 protein transfers NEDD8 from NEDD8-conjugated E2 to the cullin-RBX complex and regulates SCF-type complex activity. DCUN1D2 Protein, Human (His) is the recombinant human-derived DCUN1D2 protein, expressed by E. coli , with N-His labeled tag.

DCUN1D2 蛋白在所有 cullin 的 neddy 化过程中发挥着至关重要的作用，促进 NEDD8 从 N 端乙酰化的 NEDD8 缀合 E2 酶转移到各种 cullin C 端结构域-RBX 复合物。该功能对于调节 SCF（SKP1-CUL1-F-box 蛋白）型复合物的活性至关重要。通过其 DCUN1 结构域，DCUN1D2 与未内基化的 cullin 相互作用，包括 CUL1、CUL2、CUL3、CUL4A、CUL4B 和 CUL5。这些相互作用的特异性受到库林特性的影响，决定了相互作用的亲和力。DCUN1D2 还可能与 cullin-RING 连接酶的调节子或亚基结合，例如 RBX1、RNF7、ELOB 和 DDB1，这些相互作用由 cullin 介导。此外，DCUN1D2 与 CAND1 形成复合物，抑制 CUL3 的 neddylation。值得注意的是，CAND1-cullin-DCNL 复合物仅在底物接头存在的情况下才会发生 neddylation。此外，DCUN1D2 与 UBE2M 和 UBE2F 的 N 末端乙酰化形式相互作用，突显其参与 neddylation 途径中复杂的蛋白质-蛋白质相互作用。

Human

E. coli

N-6*His

Q6PH85-1 (M1-F259)

55208  [NCBI]

MHKLKSSQKDKVRQFMACTQAGERTAIYCLTQNEWRLDEATDSFFQNPDSLHRESMRNAVDKKKLERLYGRYKDPQDENKIGVDGIQQFCDDLSLDPASISVLVIAWKFRAATQCEFSRKEFLDGMTELGCDSMEKLKALLPRLEQELKDTAKFKDFYQFTFTFAKNPGQKGLDLEMAVAYWKLVLSGRFKFLDLWNTFLMEHHKRSIPRDTWNLLLDFGNMIADDMSNYDEEGAWPVLIDDFVEYARPVVTGGKRSLF

Approximately 30 kDa

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  Greater than 90% as determined by reducing SDS-PAGE.

溶液

Lyophilized from a 0.2 μm filtered solution of PBS, 20% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.