DDC 蛋白, Mouse (sf9, His)

The DDC protein plays a key role in neurotransmitter synthesis as it catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to produce dopamine and L-5-hydroxytryptophan to produce serotonin. These enzymatic reactions represent key steps in neurotransmitter biosynthesis and are essential for the normal functioning of the central nervous system. DDC Protein, Mouse (sf9, His) is the recombinant mouse-derived DDC protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of DDC Protein, Mouse (sf9, His) is 480 a.a., with molecular weight of ~55 kDa.

DDC，即芳香族L-氨基酸脱羧酶，是一种催化L-3,4-二羟基苯丙氨酸（DOPA）脱羧产生多巴胺和L-5-羟色氨酸产生血清素的酶。这种酶活性是神经递质生物合成的关键步骤，特别是多巴胺（一种参与情绪调节和运动控制等各种生理过程的神经递质）和血清素（对于情绪和情绪调节至关重要）的合成的关键步骤。DDC 介导的脱羧反应对于这些神经递质的可用性是不可或缺的，强调了该酶在神经化学途径中的重要性。它必须强调 DDC 在前体转化为神经递质中的特定作用，强调其在神经递质生物合成中的重要性。

Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1500 pmol/min/µg, as measured under the described conditions.

Mouse

Sf9 insect cells

C-His

O88533 (M1-E480)

13195  [NCBI]

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as 0.22 μm filtered solution in 20 mM Tris, 0.3 M NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.