Delta-like 蛋白 1/DLL1 蛋白, Mouse (HEK293, C-His)

DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces through clathrin-mediated endocytosis, involving ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-His labeled tag.

Delta 样蛋白 1 (DLL1) 是 NOTCH1、NOTCH2 和 NOTCH3 受体的跨膜配体，与 Notch 受体的胞外结构域 (ECD) 进行顺式和反式相互作用。反式相互作用后，表达 DLL1 的细胞通过网格蛋白介导的内吞作用产生机械力，需要配体泛素化、EPN1 相互作用和肌动蛋白聚合。这些事件诱导Notch受体胞外域(NECD)转内吞作用，激活Notch信号传导，包括Notch受体(NICD)胞内域的裂解、过度磷酸化和核积累。DLL1 对于胚胎发育以及跨各种组织和免疫系统的成体干细胞的维持至关重要，协调细胞间通讯以调节细胞谱系、规格、模式和形态发生，影响分化和增殖。在大脑发育中，DLL1 调节神经元分化和新皮质发育，而在小脑发育中，它影响伯格曼神经胶质单层的形成。DLL1 通过 Notch 信号通路促进免疫系统发育、肌肉发育、胰腺细胞发育、动脉特性维持、血管生成、杯状细胞分化、内耳发育和肾单位发育。DLL1 作为同二聚体发挥作用，并与各种蛋白质相互作用，在细胞过程中发挥不同的作用。

Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.4845-0.6340 μg/mL.

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  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is approximately 0.6340 μg/mL, corresponding to a specific activity is 1577.2871 units/mg.

Mouse

HEK293

C-10*His

Q61483 (Q18-Q516)

13388

QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGSGPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGAGIDPAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLTTQRHLTVGEEWSQDLHSSGRTDLRYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGDRGEKMCDPGWKGQYCTDPICLPGCDDQHGYCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCRNGATCTNTGQGSYTCSCRPGYTGANCELEVDECAPSPCKNGASCTDLEDSFSCTCPPGFYGKVCELSAMTCADGPCFNGGRCSDNPDGGYTCHCPLGFSGFNCEKKMDLCGSSPCSNGAKCVDLGNSYLCRCQAGFSGRYCEDNVDDCASSPCANGGTCRDSVNDFSCTCPPGYTGKNCSAPVSRCEHAPCHNGATCHQRGQRYMCECAQGYGGPNCQ

Approximately 58-60 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM HEPES, 150 mM NaCl, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.