Delta-like 蛋白 4/DLL4 蛋白, Mouse (HEK293, His)

Delta-like 4/DLL4 protein is a key player in the Notch signaling pathway, acting as a ligand to activate NOTCH1 and NOTCH4. In angiogenesis, DLL4 regulates endothelial cell proliferation, migration, and angiogenic sprouting. Delta-like protein 4/DLL4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Delta-like protein 4/DLL4 protein, expressed by HEK293 , with C-His labeled tag. The total length of Delta-like protein 4/DLL4 Protein, Mouse (HEK293, His) is 525 a.a., with molecular weight of ~68 kDa.

Delta 样蛋白 4/DLL4 蛋白在 Notch 信号通路中发挥着关键作用，作为 Notch 配体并激活 NOTCH1 和 NOTCH4。在血管生成的背景下，DLL4 通过负面影响内皮细胞增殖、迁移和血管生成萌芽而发挥调节作用。该蛋白质对于视网膜祖细胞增殖至关重要，并且是抑制晚期视网膜祖细胞中视杆细胞命运所必需的，同时有助于其他视网膜细胞类型的正确生成。此外，在脊髓神经发生过程中，DLL4 抑制 V2a 中间神经元的命运。它与 NOTCH4 和 NOTCH1 的相互作用突出了其作为 Notch 信号级联中配体的分子基础，强调了其在协调发育过程中关键细胞过程中的多方面功能。

1.Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED₅₀ for this effect is typically 1-5 µg/mL in the presence of 500 ng/mL recombinant human BMP2.
2.Immobilized DLL4 Protein, Mouse, Recombinant (His Tag) at 2 μg/mL (100 μL/well) can bind NOTCH1 Protein, Mouse, Recombinant (ECD, hFc Tag),the EC₅₀ is 5-30 ng/mL.

Mouse

HEK293

C-His

Q9JI71 (S28-P525)

54485  [NCBI]

Approximately 68 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.