NS1 蛋白, Dengue virus 1 (HEK293, His)

The NS1 protein plays a key role in viral budding, association with cell membranes, and facilitating the assembly of viral RNA into nucleocapsids to form mature viral particles.During entry, NS1 induces genome penetration into the host cytoplasm.NS1 Protein, Dengue virus 1 (HEK293, His) is the recombinant Virus-derived NS1 protein, expressed by HEK293 , with N-His labeled tag.

NS1 蛋白通过与细胞膜结合，促进病毒 RNA 组装成核衣壳，从而形成成熟病毒颗粒的核心，从而在病毒出芽中发挥关键作用。在病毒进入过程中，NS1 可能会在表面蛋白诱导的半融合后诱导基因组渗透到宿主细胞质中。值得注意的是，NS1 表现出迁移到细胞核的能力，在细胞核中调节宿主功能。此外，NS1 通过蛋白酶体降解途径隔离和降解 EXOC1，抵消宿主 EXOC1 的抗病毒作用。此外，NS1 通过干扰宿主 Dicer 来破坏 RNA 沉默，突显其在整个感染周期中操纵病毒和宿主细胞过程中的多方面作用。

Virus

HEK293

N-His

ACF49259 (D776-A1127)

/

Approximately 42.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.