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  3. Der p 23 蛋白, Dermatophagoides pteronyssinus (HEK293, His)

Der p 23 蛋白, Dermatophagoides pteronyssinus (HEK293, His)

目录号: HY-P75289
COA 产品使用指南

Der p 23 蛋白被鉴定为过敏性房尘螨蛋白的成员,并且在体外不表现出几丁质结合能力。这一独特的功能将 Der p 23 与其他因其与几丁质的亲和力而闻名的蛋白质区分开来,强调了其独特的特性。Der p 23 蛋白, Dermatophagoides pteronyssinus (HEK293, His) 是重组的 Der p 23 蛋白,由 HEK293 表达,带有 C-His 标签。Der p 23 蛋白, Dermatophagoides pteronyssinus (HEK293, His) 全长 69 个氨基酸,分子量约为 ~19 kDa。

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描述

The Der p 23 protein was identified as a member of the allergic house dust mite proteins and does not exhibit chitin-binding ability in vitro. This unique feature distinguishes Der p 23 from other proteins known for their affinity to chitin, emphasizing its unique properties. Der p 23 Protein, Dermatophagoides pteronyssinus (HEK293, His) is the recombinant Der p 23 protein, expressed by HEK293 , with C-His labeled tag. The total length of Der p 23 Protein, Dermatophagoides pteronyssinus (HEK293, His) is 69 a.a., with molecular weight of ~19 kDa.

研究背景

根据现有信息,Der p 23 蛋白在体外不表现出几丁质结合活性。几丁质是一种多糖,通常与昆虫的外骨骼和真菌的细胞壁相关,Der p 23 中不存在几丁质结合表明其具有独特的功能特征。此外,Der p 23 被描述为单体蛋白,这意味着它作为单个独立单元存在,而不是与其他分子形成复合物。了解这些特征对于阐明 Der p 23 的生物学作用至关重要,特别是在其与环境因素相互作用的背景下,需要进一步研究来描述该蛋白质在相关生理背景下的具体功能和影响。(参考资料:根据现有资料改编,未注明具体出处)

种属

Others

表达系统

HEK293

标签

C-His

蛋白编号

L7N6F8 (A22-T90)

基因 ID

113788516  [NCBI]

蛋白结构
N-term
DEP23 (A22-T90)
Accession # L7N6F8
His
C-term
中文名
重组螨Der p 23蛋白
同用名
Major mite allergen Der p 23; Peritrophin-like protein Der p 23; Der p 23
氨基酸序列

ANDNDDDPTTTVHPTTTEQPDDKFECPSRFGYFADPKDPHKFYICSNWEAVHKDCPGNTRWNEDEETCT

分子量

Approximately 19 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Der p 23 Protein, Dermatophagoides pteronyssinus (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Der p 23 Protein, Dermatophagoides pteronyssinus (HEK293, His)
目录号:
HY-P75289
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