DERP5 蛋白, Dermatophagoides pteronyssinus (His)

DERP5 protein exhibits a monomeric structure and forms homodimeric trimers through concentration-dependent oligomerization, which plays a key role in allergic reactions. Multiple population studies have shown that DERP5 binds to IgE and is associated with mite allergy symptoms such as asthma and rhinitis. DERP5 Protein, Dermatophagoides pteronyssinus (His) is the recombinant DERP5 protein, expressed by E. coli , with N-His labeled tag. The total length of DERP5 Protein, Dermatophagoides pteronyssinus (His) is 132 a.a., with molecular weight of ~16.6 KDa.

DERP5蛋白呈现单体结构，并进一步形成同二聚体的三聚体，以浓度依赖性方式寡聚化。值得注意的是，DERP5 与引起人类过敏反应有关，螨虫过敏的常见症状包括支气管哮喘、过敏性鼻炎和结膜炎。多项研究证明，它与对屋尘螨 (HDM) 过敏的个体中的 IgE 结合。重组 DERP5 蛋白在不同人群中测试的相当大比例的患者中显示出 IgE 结合。此外，重组蛋白的皮肤测试在哮喘和过敏性鼻炎患者中显示出阳性反应。重要的是，DERP5 不表现出与 Der p 21 过敏原的相关交叉反应性。此外，DERP5 诱导人外周血单核白细胞释放组胺，并上调嗜碱性粒细胞上 CD203c 激活标记物的表达。这些发现强调了 DERP5 在过敏反应中的作用及其在理解和管理螨虫引起的过敏性疾病方面的潜在意义。

Others

E. coli

N-His

P14004 (M1-V132)

113793750  [NCBI]

MKFIIAFFVATLAVMTVSGEDKKHDYQNEFDFLLMERIHEQIKKGELALFYLQEQINHFEEKPTKEMKDKIVAEMDTIIAMIDGVRGVLDRLMQRKDLDIFEQYNLEMAKKSGDILERDLKKEEARVKKIEV

Approximately 15 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.