DKK-3 蛋白, Human (R335G, HEK293, His)

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interactions and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization.In vertebrate development, DKK-3, together with other DKKs, critically inhibits Wnt-regulated processes such as axial patterning, limb development, somitogenesis, and eye formation.DKK-3 Protein, Human (R335G, HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag and R335G, , , , mutation.

DKK-3 蛋白通过阻止 LRP5/6 和 Wnt 之间的相互作用并与跨膜蛋白 KREMEN 形成三元复合物，从而促进 LRP5/6 的内化，从而充当经典 Wnt 信号传导的拮抗剂。在脊椎动物发育中，DKK（包括 DKK-3）通过局部抑制 Wnt 调节过程（例如前后轴模式、肢体发育、体节发生和眼睛形成）发挥着至关重要的作用。在成人中，DKK 与多种生理和病理状况有关，包括骨形成、骨疾病、癌症和阿尔茨海默氏病（通过相似性）。值得注意的是，DKK-3 与 LRP5 和 LRP6 的相互作用凸显了其在抑制 Wnt 信号传导中的调节作用及其对各种细胞过程的潜在影响。

Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED₅₀ for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

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  Measured by its ability to inhibit the proliferation of Hela human cervical carcinoma cell. The ED₅₀ for this effect is 3.621 μg/mL, corresponding to a specific activity is 276.167 units/mg.

Human

HEK293

C-6*His

Q9UBP4 (A22-I350, R335G)

27122  [NCBI]

APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI

50-66 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.