DNA helicase II/uvrD 蛋白, E.coli

DNA helicase II/uvrD Protein, E.coli encodes an 82-kDa protein with both ATPase and helicase activities. DNA helicase II/uvrD Protein, E.coli unwinds DNA duplexes with 3' to 5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. DNA helicase II/uvrD Protein, E.coli is involved in methyl-directed mismatch repair and nucleotide excision repair of DNA^[1][2][3] DNA helicase II/uvrD Protein, E.coli is the recombinant E. coli-derived DNA helicase II/uvrD protein, expressed by E. coli , with tag free. The total length of DNA helicase II/uvrD Protein, E.coli is 720 a.a., with molecular weight of ~82.0 kDa.

DNA helicase II/uvrD Protein 也可以在较高酶浓度下从切口或平端开始解旋。DNA helicase II/uvrD Protein 参与核苷酸切除修复和甲基定向错配修复的切割后事件^[1]。
DNA helicase II/uvrD Protein 突变体对 UV 和 Azidothymidine (AZT; HY-17413) 强烈敏感，对 CPX 中度敏感，并且通过添加 radA 突变可增强敏感性。在recF中引入突变几乎完全抑制了uvrD和uvrD radA突变体的AZT敏感性并部分抑制了UV和CPX敏感性^[2]。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

E.coli

E. coli

Tag Free

P03018 (M1-V720)

948347  [NCBI]

MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV

Approximately 82.0 kDa

• 
  Greater than 91% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from 0.22 μm filtered solution in PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.