DNMT2 蛋白, Human (sf9, GST)

The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of DNMT2 Protein, Human (sf9, GST) is 391 a.a., with molecular weight of ~60 kDa.

DNMT2 蛋白在其酶活性方面表现出显着的特异性，因为它选择性地甲基化 tRNA (Asp) 反密码子环内的胞嘧啶 38。这种位点特异性甲基化事件在转移 RNA 分子的表观遗传修饰中发挥着至关重要的作用，可能影响它们的结构、稳定性和功能相互作用。通过靶向 tRNA (Asp) 反密码子环中的特定胞嘧啶残基，DNMT2 有助于调节翻译效率和保真度。该酶对该特定位点甲基化的精确性强调了其在控制 tRNA 功能的复杂调节机制中的重要性，强调了 DNMT2 在涉及 tRNA(Asp) 的细胞过程的表观遗传景观中的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

O14717 (M1-E391)

1787  [NCBI]

Approximately 60 kDa(Predicted Molecular Mass :70.88 kDa)

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM GSH, 10% Glycerol, pH 8, 5% Trehalose, 5% Mannitol, 0.01% Tween-80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.