1. 重组蛋白
  2. Enzymes & Regulators
  3. Oxidoreductases (EC 1)
  4. Dopamine beta-Hydroxylase 蛋白, Human (HEK293, His)

Dopamine beta-Hydroxylase 蛋白, Human (HEK293, His)

目录号: HY-P76874
COA 产品使用指南

多巴胺β-羟化酶蛋白是参与神经递质调节的重要酶。其主要作用是催化多巴胺的羟基化,将其转化为去甲肾上腺素(又称去甲肾上腺素)。Dopamine beta-Hydroxylase 蛋白, Human (HEK293, His) 是重组的 多巴胺 β-羟化酶蛋白,由 HEK293 表达,带有 N-His 标签。Dopamine beta-Hydroxylase 蛋白, Human (HEK293, His) 全长 578 个氨基酸,分子量约为 ~68 kDa。

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规格 价格 是否有货 数量
50 μg ¥4600 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Dopamine beta-hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its main function is to catalyze the hydroxylation of dopamine, converting it into norepinephrine (also known as norepinephrine). Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is the recombinant human-derived Dopamine beta-Hydroxylase protein, expressed by HEK293 , with N-His labeled tag. The total length of Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is 578 a.a., with molecular weight of ~68 kDa.

研究背景

多巴胺β-羟化酶蛋白是参与神经递质调节的必需酶。其主要功能是催化多巴胺的羟基化,将其转化为去甲肾上腺素(也称为去甲肾上腺素)。这种酶活性对于多巴胺转化为去甲肾上腺素至关重要,在调节神经系统内的这些神经递质方面发挥着重要作用。通过促进这种化学转化,多巴胺β-羟化酶蛋白有助于调节神经递质水平并影响体内的各种生理过程。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Human

表达系统

HEK293

标签

N-His

蛋白编号

P09172 (S26-G603)

基因 ID
蛋白结构
N-term
His
DBH (S26-G603)
Accession # P09172
C-term
同用名
Dopamine beta-hydroxylase; DBH; DOPBHY
分子量

Approximately 68 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Dopamine beta-Hydroxylase Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Dopamine beta-Hydroxylase Protein, Human (HEK293, His)
目录号:
HY-P76874
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