1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. DOT1L 蛋白, Human

DOT1L 蛋白, Human

目录号: HY-P75283
产品使用指南

DOT1L 蛋白是一种组蛋白甲基转移酶,可将组蛋白 H3 的赖氨酸 79 甲基化。它对核小体表现出高活性,但对游离核心组蛋白没有表现出高活性。它广泛表达,特别是在睾丸、骨髓和 22 种其他组织等组织中。DOT1L 蛋白, Human 是重组的 DOT1L 蛋白,由 E. coli 表达,不带标签。

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规格 是否有货
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100 μg   询价  

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描述

The DOT1L protein, a histone methyltransferase, methylates lysine-79 of histone H3. It shows high activity against nucleosomes but not free core histones. It is broadly expressed, particularly in tissues like the testis, bone marrow, and 22 other tissues. DOT1L Protein, Human is the recombinant human-derived DOT1L protein, expressed by E. coli , with tag free.

研究背景

DOT1L 蛋白是一种组蛋白甲基转移酶,负责组蛋白 H3 的赖氨酸 79 甲基化。虽然它对游离核心组蛋白没有表现出显着的活性,但它对核小体表现出显着的组蛋白甲基转移酶活性。这种蛋白质广泛表达,在睾丸、骨髓和 22 种其他组织等组织中表达显着。

种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

Q8TEK3-1/NP_115871.1 (G2-K416)

基因 ID
蛋白结构
N-term
DOT1L (G2-K416)
Accession # Q8TEK3-1/NP_115871.1
C-term
同用名
Histone H3-K79 methyltransferase; H3-K79-HMTase; DOT1L; KIAA1814; KMT4
分子量

Approximately 50 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

DOT1L Protein, Human 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
DOT1L Protein, Human
目录号:
HY-P75283
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