1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. DPYS 蛋白, Human (sf9)

DPYS 蛋白, Human (sf9)

目录号: HY-P75713
产品使用指南

DPYS 蛋白通过催化涉及二氢嘧啶可逆水解的第二步,在还原嘧啶降解途径中发挥关键作用。其酶活性延伸至5,6-二氢尿嘧啶的开环,导致N-氨基甲酰基-丙氨酸的形成,以及5,6-二氢胸腺嘧啶转化为N-氨基甲酰基-氨基异丁烯酸酯。DPYS 蛋白, Human (sf9) 是重组的 DPYS 蛋白,由 Sf9 insect cells 表达,不带标签。DPYS 蛋白, Human (sf9) 全长 519 个氨基酸,分子量约为 ~54 kDa。

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描述

The DPYS protein plays a key role in the reductive pyrimidine degradation pathway by catalyzing the second step involving the reversible hydrolysis of dihydropyrimidines. Its enzymatic activity extends to the ring opening of 5,6-dihydrouracil, leading to the formation of N-carbamoyl-alanine, and the conversion of 5,6-dihydrothymine to N-carbamoyl-aminoisobutylene acid ester. DPYS Protein, Human (sf9) is the recombinant human-derived DPYS protein, expressed by Sf9 insect cells , with tag free. The total length of DPYS Protein, Human (sf9) is 519 a.a., with molecular weight of ~54 kDa.

研究背景

二氢嘧啶酶(DPYS)是一种在还原嘧啶降解途径中发挥关键作用的酶,催化二氢嘧啶的可逆水解开环。具体来说,DPYS可以催化5,6-二氢尿嘧啶开环生成N-氨甲酰-丙氨酸和5,6-二氢胸腺嘧啶开环生成N-氨甲酰-氨基异丁酸酯。这种酶活性是嘧啶衍生物分解代谢的关键步骤,有助于核苷酸和核酸代谢物的分解。DPYS 在二氢嘧啶水解开环中的多功能性凸显了其在维持嘧啶稳态和细胞内嘧啶相关化合物的循环中的重要性。了解 DPYS 的功能有助于深入了解嘧啶代谢的调节及其对细胞过程的影响。参考资料:所提供的信息基于截至 2022 年 1 月的一般知识,未引用具体参考资料。

种属

Human

表达系统

Sf9 insect cells

标签

Tag Free

蛋白编号

Q14117 (N-G&P, M1-P519)

基因 ID
蛋白结构
N-term
Gly-Pro
DPYS (M1-P519)
Accession # Q14117
C-term
同用名
Dihydropyrimidinase; DHP; DHPase; Hydantoinase; DPYS
分子量

Approximately 54 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

DPYS Protein, Human (sf9) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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