dUTPase/DUT 蛋白, Human

Deoxyuridine 5'-triphosphate nucleotidohydrolase (DUT) is an essential enzyme of nucleotide metabolism, as DUT hydrolyzes dUTP to dUMP and pyrophosphate, preventing uracil misincorporation into DNA efficiently and provides dUMP for thymidylate biosynthesis. DUT also prevents dimerization of PPAR and retinoid X receptor and is essential for embryonic development as well. dUTPase/DUT Protein, Human is the recombinant human-derived dUTPase/DUT protein, expressed by E. coli , with tag free. The total length of dUTPase/DUT Protein, Human is 164 a.a., with molecular weight of ~18.0 kDa.

脱氧尿苷 5'-三磷酸核苷酸水解酶 (DUT) 是核苷酸代谢的必需酶，它形成一种普遍存在的同源四聚体酶，将 dUTP 水解为 dUMP 和焦磷酸，有效地防止尿嘧啶错误掺入 DNA，同时提供 dUMP 这一从头合成胸苷酸的底物。DUT 的可变剪切产生定位于线粒体或细胞核的不同亚型。
DUT 还通过将 N 端与过氧化物酶体增殖物激活受体 (PPAR) 结合，阻止 PPAR 与维甲酸 X 受体二聚化，从而抑制 PPAR 活性。DUT 对胚胎发育也至关重要^[1][2][3]。

Measured by its ability to catalyze the substrate dUTP. The specific activity is 65400.467 pmol/min/mg, as measured under the described conditions.

Human

E. coli

Tag Free

P33316-2 (M1-N164)

1854  [NCBI]

MPCSEETPAISPSKRARPAEVGGMQLRFARLSEHATAPTRGSARAAGYDLYSAYDYTIPPMEKAVVKTDIQIALPSGCYGRVAPRSGLAAKHFIDVGAGVIDEDYRGNVGVVLFNFGKEKFEVKKGDRIAQLICERIFYPEIEEVQALDDTERGSGGFGSTGKN

Approximately 18.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.