E-Selectin/CD62E 蛋白, Human (HEK293, His-Fc)

E-selectin/CD62E Protein, a cell-surface glycoprotein, crucially mediates immunoadhesion by interacting with SELPLG/PSGL1 for the adhesion of neutrophils to cytokine-activated endothelium. It also potentially influences capillary morphogenesis and interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope, independently of SELPLG sulfation. E-Selectin/CD62E Protein, Human (HEK293, His-Fc) is the recombinant human-derived E-Selectin/CD62E protein, expressed by HEK293 , with C-hFc, C-His labeled tag. The total length of E-Selectin/CD62E Protein, Human (HEK293, His-Fc) is 535 a.a., with molecular weight of 140-150 kDa.

E-选择素/CD62E 蛋白是一种细胞表面糖蛋白，在免疫粘附中发挥着至关重要的作用。它通过与 SELPLG/PSGL1 相互作用介导血液中性粒细胞在细胞因子激活的内皮细胞中的粘附。此外，E-选择素/CD62E 蛋白可能参与毛细血管形态发生。它通过唾液酸路易斯 X 表位与 SELPLG/PSGL1 和 PODXL2 相互作用，值得注意的是，SELPLG 硫酸化对于这种相互作用不是必需的。

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When 5 x 10⁴ cells/well are added to mouse E-Selectin/Fc Chimera coated plates (2 µg/mL with 100 µL/well), approximately 95.30% will adhere for 1 hour incubation at room temperature.

Human

HEK293

C-hFc;C-His

P16581 (W22-P556)

6401  [NCBI]

WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYWIGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKDVGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSGLKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMETMQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFDCEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSPAGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTALSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWDNEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHGSTQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFACPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP

140-150 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.