EB3/MAPRE3 蛋白, Human (His)

The EB3/MAPRE3 protein is a plus-end tracking protein (+TIP) that dynamically regulates microtubule cytoskeletal dynamics by binding to the plus-end of microtubules. It promotes microtubule growth, stabilizes it at the centrosome, and regulates minus-end microtubule organization. EB3/MAPRE3 Protein, Human (His) is the recombinant human-derived EB3/MAPRE3 protein, expressed by E. coli , with N-His labeled tag.

EB3 是一种正端追踪蛋白 (+TIP)，通过与微管正端结合来复杂地调节微管细胞骨架的动力学。从功能上来说，EB3 可以作为微管生长的促进剂，并通过稳定微管并将其锚定在中心体来潜在地促进纺锤体功能。此外，它还是负端微管组织的重要调节因子，通过与 AKAP9 和 PDE4DIP 形成的复合物相互作用，参与将 CAMSAP2 招募到高尔基体。这种相互作用对于将非中心体负端微管束缚到高尔基体至关重要，这是极化细胞运动所必需的过程。EB3 与 MAPRE1 形成同二聚体和异二聚体，结合单体和聚合的 GTP 结合微管蛋白。其复杂的相互作用网络包括 APC2、DCTN1、SRCIN1、CLIP1、SLAIN2、SLAIN1、AKAP9 和 PDE4DIP，强调了其在微管动力学和细胞组织中的多方面作用。

Human

E. coli

N-6*His

Q9UPY8-1 (M1-Y281)

22924  [NCBI]

MAVNVYSTSVTSENLSRHDMLAWVNDSLHLNYTKIEQLCSGAAYCQFMDMLFPGCVHLRKVKFQAKLEHEYIHNFKVLQAAFKKMGVDKIIPVEKLVKGKFQDNFEFIQWFKKFFDANYDGKDYNPLLARQGQDVAPPPNPGDQIFNKSKKLIGTAVPQRTSPTGPKNMQTSGRLSNVAPPCILRKNPPSARNGGHETDAQILELNQQLVDLKLTVDGLEKERDFYFSKLRDIELICQEHESENSPVISGIIGILYATEEGFAPPEDDEIEEHQQEDQDEY

Approximately 34 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.