EBP50/SLC9A3R1 蛋白, Human (HEK293, His)

EBP50 is a scaffolding protein that connects plasma membrane proteins to the actin cytoskeleton, regulates its surface expression and forms homo- or heterodimers with NHERF2. It regulates the activity and localization of SLC9A3 and is critical for the recycling of ADRB2. EBP50/SLC9A3R1 Protein, Human (HEK293, His) is the recombinant human-derived EBP50/SLC9A3R1, expressed by HEK293 , with C-6*His labeled tag.

EBP50 作为支架蛋白，促进质膜蛋白与埃兹蛋白/模蛋白/根蛋白家族成员之间的连接，从而将它们与肌动蛋白细胞骨架连接并调节其表面表达。EBP50 对于内化 ADRB2 的循环至关重要，在 SLC9A3 活性和亚细胞定位的调节中发挥着至关重要的作用。它对于 cAMP 介导的磷酸化和 SLC9A3 的抑制是必不可少的，并且可能有助于 Wnt 信号传导和 HTR4 靶向微绒毛。此外，EBP50 还参与肾近曲小管的磷酸盐重吸收、精子获能以及精子中氯离子和碳酸氢盐稳态的调节。它与 NHERF2 形成同二聚体和异二聚体，并与多种蛋白质相互作用，包括 ezrin、radixin、moesin、PDGFRA、PDGFRB、ADRB2、NOS2、CFTR、ARHGAP17、EPI64、RACK1、OPRK1、GNAQ、CTNNB1、PLCB3 和 PDZK1。此外，EBP50 参与与 CLCN3、PAG1、CFTR、SLC4A7、ATP6V1B1、TRPC4、HTR4、PODXL、SLC26A3、MCC、SLC34A1、SLC26A6 和 ACE2 的复合物，突出了其在细胞过程和信号传导途径中的多种作用。

Human

HEK293

C-6*His

O14745-1 (M1-L358)

9368

MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL

Approximately 48-60 kDa due to the phosphorylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.