Ecotin 蛋白, E. coli (His)

Ecotin, a bacterial protein inhibitor, regulates serine proteases like trypsin and subtilisin. Ecotin's unique surface loop forms a stable complex, influencing enzymatic activity. Ecotin Protein, E. coli (His) is the recombinant E. coli-derived Ecotin protein, expressed by E. coli , with C-His labeled tag. The total length of Ecotin Protein, E. coli (His) is 142 a.a., with molecular weight of ~18 kDa.

Ecotin 是一种蛋白质抑制剂，可调节细菌中的蛋白酶活性。它特异性抑制多种丝氨酸蛋白酶，包括胰蛋白酶和枯草杆菌蛋白酶。这种调节作用使细菌能够控制蛋白酶介导的过程，影响各种细胞功能。Ecotin 与蛋白酶的相互作用涉及独特的表面环，该环形成稳定的复合物，影响酶活性。这种蛋白质在细菌生理学中起着至关重要的作用，并且对研究蛋白酶抑制机制很有意义。

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The IC50 value is <1.4 nM.

E.coli

E. coli

C-His

NP_416713 (A21-R162)

946700  [NCBI]

AESVQPLEKIAPYPQAEKGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAVVR

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of Tris and NaCl or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.