EDAR 蛋白, Rat (HEK293, Fc)

EDAR Protein is a typical Tumor Necrosis Factor receptor (TNFR) family member. EDAR is a receptor for EDA isoform A1. Its binding results in recruitment of the intracellular EDAR-associated death domain (EDARADD) adapter protein and simultaneous activation of NF-kappa-B and JNK signaling pathways, potentially leading to various cellular responses. Additionally, EDAR may play a role in promoting caspase-independent cell death. EDAR Protein, Rat (HEK293, Fc) is the recombinant rat-derived EDAR protein, expressed by HEK293 , with C-hFc labeled tag. The total length of EDAR Protein, Rat (HEK293, Fc) is 161 a.a., with molecular weight of 53-75 kDa.

外胚层发育蛋白-A 受体 (EDAR) 是典型的肿瘤坏死因子受体 (TNFR) 家族成员。EDAR 是一种专门针对 EDA 同工型 A1 的受体，与 EDA 同工型 A2 区别开来。EDA-A1/EDAR 结合导致细胞内 EDAR 相关死亡结构域 (EDARADD) 接头蛋白的募集，并同时激活 NF-kappa-B 和 JNK 信号通路，可能导致各种细胞反应。此外，EDAR 可能在促进不依赖 caspase 的细胞死亡中发挥作用。该受体与 EDARADD 形成复合物，并与 TRAF1、TRAF2、TRAF3 和 NIK 等关键信号分子相关，表明其参与复杂的信号级联反应。此外，EDAR 通过诱导 Wnt/β-catenin 信号传导促进肿瘤细胞增殖^[1]。

Measured by its binding ability in a functional ELISA. Immobilized Rat EDAR at 2 μg/mL (100 μL/well) can bind Anti-EDAR antibody, The ED₅₀ for this effect is 408.3ng/mL .

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  Measured by its binding ability in a functional ELISA. Immobilized Rat EDAR at 2 μg/mL (100 μL/well) can bind Anti-EDAR antibody, The ED₅₀ for this effect is 408.3ng/mL .

Rat

HEK293

C-hFc

D3ZGP2/NP_001178828.1 (E27-A187)

365581  [NCBI]

EDSNCGENEYHNQTTGLCQQCPPCRPGEEPYMSCGYGTKDEDYGCVPCPAEKFSKGGYQICRRHKDCEGFFRATVLTPGDMENDAECGPCLPGYYMLENRPRNIYGMVCYSCLLAPPNTKECVGATSGVSAHSSSTSGGSTLSPFQHAHKELSSQGHLATA

Approximately 53-75 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.