EEF1E1 蛋白, Human (His)

The EEF1E1 protein is an important component of a multi-subunit complex that actively regulates the ATM response to DNA damage. It coordinates tRNA ligases of various amino acids and interacts with both MARS1 and EPRS1 in a core complex with AIMP2. EEF1E1 Protein, Human (His) is the recombinant human-derived EEF1E1 protein, expressed by E. coli , with N-His labeled tag. The total length of EEF1E1 Protein, Human (His) is 174 a.a., with molecular weight of 19-24 KDa.

EEF1E1 是 ATM 对 DNA 损伤反应的正调节剂，是多亚基复合物的重要组成部分，该复合物协调 Arg (RARS1)、Asp (DARS1)、Gln (QARS1)、Ile (IARS1)、Leu (LARS1) 的 tRNA 连接酶、Lys (KARS1)、Met (MARS1) 和 Glu 和 Pro (EPRS1) 双功能连接酶，以及辅助亚基 AIMP1/p43、AIMP2/p38 和 EEF1E1/p18。它形成一个以 MARS1、EEF1E1、EPRS1 和 AIMP2 为核心的线性复合体。EEF1E1 表现出与 MARS1 和 EPRS1 同时相互作用，突出了其在多亚基复合物中的不可或缺的作用。此外，EEF1E1 与 ATM 和 ATR 相互作用。与 ATM 的相互作用独立于 TP53，由基因毒性应激或细胞生长引起的 DNA 损伤诱导，而与 ATR 的相互作用因紫外线照射而增强。这个复杂的网络强调了 EEF1E1 在协调 DNA 损伤反应机制中的重要性。

Human

E. coli

N-His

O43324 (M1-H174)

9521  [NCBI]

MAAAAELSLLEKSLGLSKGNKYSAQGERQIPVLQTNNGPSLTGLTTIAAHLVKQANKEYLLGSTAEEKAIVQQWLEYRVTQVDGHSSKNDIHTLLKDLNSYLEDKVYLTGYNFTLADILLYYGLHRFIVDLTVQEKEKYLNVSRWFCHIQHYPGIRQHLSSVVFIKNRLYTNSH

Approximately 22 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.