EGF 蛋白, Canine (His)

The EGF protein plays a key role in promoting the growth of a variety of epidermal and epithelial tissues in vivo and in vitro and in stimulating the proliferation of certain fibroblasts in cell culture. In addition, it also has the effect of magnesium-stimulating hormone, causing magnesium reabsorption in the renal distal convoluted tubule through the participation of EGFR and the activation of magnesium channel TRPM6. EGF Protein, Canine (His) is the recombinant canine-derived EGF protein, expressed by E. coli , with N-His labeled tag. The total length of EGF Protein, Canine (His) is 52 a.a., with molecular weight of 16-22 kDa.

表皮生长因子 (EGF) 是体内和体外各种表皮和上皮组织以及细胞培养中的一些成纤维细胞生长的有效刺激剂。这种多功能蛋白质在细胞过程中发挥着至关重要的作用，特别是在促进组织的增殖和发育方面。除了在组织生长中的作用外，EGF 还充当促镁激素，通过 EGFR 的参与和镁通道 TRPM6 的激活促进肾远曲小管中镁的重吸收。此外，EGF 与 EGFR 相互作用，促进 EGFR 二聚化，并与 RHBDF1 和 RHBDF2 结合，可能影响 EGF 的细胞内运输和降解途径。这些相互作用强调了 EGF 在细胞内生长和信号传导过程中复杂而多样的功能。

Immobilized Canine EGF, His Tag at 1 μg/mL (100 μl/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC₅₀ of 4.7 ng/mL determined by ELISA.

Canine

E. coli

N-His

Q9BEA0 (N973-R1024)

403657  [NCBI]

NGYRECPSSYDGYCLYNGVCMYIEAVDRYACNCVFGYVGERCQHRDLKWELR

16-22 kDa

Greater than 95% as determined by reducing SDS-PAGE or Tris-Bis PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.