EGLP/GPX5 蛋白, Pig (P.pastoris, Myc, His)

EGLP/GPX5 may constitute a protective system similar to glutathione peroxidase, protecting sperm membrane lipids from peroxidative damage. Despite the limited enzyme activity, the protective effect suggests an effect beyond enzyme function. EGLP/GPX5 Protein, Pig (P.pastoris, Myc, His) is the recombinant pig-derived EGLP/GPX5 protein, expressed by P. pastoris , with N-His, C-Myc labeled tag.

EGLP/GPX5 蛋白作为类似于谷胱甘肽过氧化物酶的保护系统的潜在组成部分出现，保护精子膜脂质免受过氧化物损伤。尽管纯化的猪酶对过氧化氢或有机氢过氧化物表现出有限的酶活性，但其保护作用表明其作用超出了酶的功能。相反，EGLP/GPX5 可以通过与脂质过氧化物结合来保护精子免受附睾中过早顶体反应的影响。这种结合作用可以阻止脂质过氧化物与磷脂酶 A2 的相互作用，从而减轻顶体反应的诱导。EGLP/GPX5 的多方面性质意味着它参与非酶机制，有助于防御过氧化物诱导的损伤，突显其在保护精子活力和功能方面的潜在意义。进一步的研究对于揭示 EGLP/GPX5 在这种保护能力中所协调的特定分子途径和相互作用至关重要。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Pig

P. pastoris

N-His;C-Myc

O18994 (N22-E219)

396920  [NCBI]

NSNLEKMDCYKDVTGTIYDYDAFTLNGNEHIQFKQYAGKHVLFVNVATYCGLTAQYPELNTLQEELKPFGLVVLGFPCNQFGKQEPGENSEILLGLKYVRPGGGYVPNFQLFEKGDVNGEKEQKVFTFLKHSCPHPSELIGSIGYISWEPIRVHDIRWNFEKFLVGPDGVPVMRWVHETPISTVKSDILAYLKQFKTE

Approximately 26.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.