EGR1/ZNF225 蛋白, Human (His)

The EGR1/ZNF225 protein is a multifunctional transcriptional regulator that binds to the EGR site in the promoter of target genes and is independent of cytosine methylation. It controls the transcription of multiple target genes, affecting responses to growth factors, DNA damage, and ischemia. EGR1/ZNF225 Protein, Human (His) is the recombinant human-derived EGR1/ZNF225 protein, expressed by E. coli , with N-6*His labeled tag. The total length of EGR1/ZNF225 Protein, Human (His) is 152 a.a., with molecular weight of ~20.0 kDa.

EGR1/ZNF225 是一种转录调节因子，在细胞中发挥多种作用，识别并结合靶基因启动子内的 DNA 序列 5'-GCG(T/G)GGGCG-3'（EGR 位点）。无论胞嘧啶甲基化状态如何，它都会结合双链靶 DNA。从功能上讲，EGR1/ZNF225 控制着众多靶基因的转录，在调节对生长因子、DNA 损伤和缺血的反应中发挥着至关重要的作用。它有助于细胞存活、增殖和死亡的复杂平衡，激活 p53/TP53 和 TGFB1 等关键调节因子的表达，以防止肿瘤形成。EGR1/ZNF225 对于部分肝切除术后有丝分裂进展和肝细胞增殖至关重要，还介导对缺血和缺氧的反应，影响炎症蛋白（如 IL1B 和 CXCL2）的表达。此外，它还参与垂体黄体生成素生物合成的调节。此外，EGR1/ZNF225 协调肝脏中 BMAL1、PER2 和 NR1D1 等时钟基因的节律表达，影响昼夜节律。它在 12-O-十四烷酰佛波醇-13-乙酸酯 (TPA) 诱导下与 SNAI1 和 SP1 的动态相互作用进一步增加了其调控网络的复杂性。

Human

E. coli

N-6*His

P18146 (Q282-S433)

1958  [NCBI]

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Approximately 20.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.