EIF3K 蛋白, Human (sf9, His-GST)

The eIF3K protein is an important component of the eukaryotic translation initiation factor 3 (eIF-3) complex and plays a crucial role in various steps of protein synthesis initiation.It stimulates recruitment of mRNA to the 43S preinitiation complex (43S PIC) and promotes AUG recognition scanning.EIF3K Protein, Human (sf9, His-GST) is the recombinant human-derived EIF3K protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

eIF3K 蛋白是真核翻译起始因子 3 (eIF-3) 复合物的组成部分，是蛋白质合成起始各个步骤所需的关键组件。该复合物与 40S 核糖体结合，促进必需因子的募集以形成 43S 起始复合物 (43S PIC)。eIF-3 复合物中的 eIF-3K 刺激 mRNA 募集至 43S PIC，并协助扫描 mRNA 进行 AUG 识别。此外，eIF-3 复合物在终止后核糖体复合物的分解和回收中发挥着关键作用，防止 40S 和 60S 核糖体亚基在启动前过早连接。值得注意的是，eIF-3K 有助于参与细胞增殖的特定 mRNA 的翻译起始，包括与细胞周期、分化和凋亡相关的 mRNA。通过不同的 RNA 茎环结合模式，eIF-3K 可以发挥翻译激活或抑制作用。eIF-3K 所属的 eIF-3 复合体由 13 个亚基组成，分为三个稳定模块（A、B 和 C），其中 eIF-3K 位于模块 C 中。与其他亚基（例如 EIF3J 和 EIF3J）的相互作用CCND3 强调了 eIF3K 所涉及的复杂调控网络，进一步深入了解其在翻译起始和细胞过程中的多方面作用。

Human

Sf9 insect cells

N-His;N-GST

Q9UBQ5 (A2-Q218)

27335  [NCBI]

Approximately 47 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM Nacl, 3 mM DTT, pH 7.4, 10% Glycerol. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.