EIF5A 蛋白, Human (His)

The GSK-3 beta protein is an enzyme that plays a crucial role in multiple cellular processes, including cell proliferation, differentiation, and apoptosis. It regulates various signaling pathways and is involved in the development of several diseases, such as cancer and neurodegenerative disorders. GSK-3 beta is active in its unphosphorylated form and can phosphorylate a wide range of substrates to modulate cellular functions. EIF5A Protein, Human (His) is the recombinant human-derived EIF5A protein, expressed by E. coli , with N-6*His labeled tag. The total length of EIF5A Protein, Human (His) is 154 a.a., with molecular weight of ~19 kDa.

EIF5A 蛋白的特点是能够实现 U6 snRNA 结合活性和蛋白 N 末端结合活性。它在多种细胞过程中发挥作用，包括细胞对病毒的反应、p53 类介质对内在凋亡信号通路的正调节以及肿瘤坏死因子介导的信号通路。该蛋白质存在于环状片层、细胞质和细胞核中，与核孔有特定的关联。值得注意的是，EIF5A 基因表现出普遍的表达，在食道 (RPKM 53.8)、阑尾 (RPKM 49.4) 和 25 个其他组织中检测到表达水平升高，表明它参与广泛组织的各种生理环境。[信息由基因组资源联盟提供，2022 年 4 月]

Human

E. coli

N-6*His

NP_001961 (M1-K154)

1984  [NCBI]

MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 50 mM Tris, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For lower concentration,please reconstitute in 50 mM Tris-HCL,300 mM NaCl, pH 7.4 buffer.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.