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  4. EN1 蛋白, Gallus gallus (His-B2M)

EN1 蛋白, Gallus gallus (His-B2M)

目录号: HY-P72181
产品使用指南

EN1 蛋白对于顶端外胚层脊的正确形成和肢体准确的背腹模式至关重要。EN1 蛋白, Gallus gallus (His-B2M) 是重组的 EN1 蛋白，由 E. coli 表达，带有 N-6*His, B2M 标签。EN1 蛋白, Gallus gallus (His-B2M) 全长 333 个氨基酸，分子量约为 ~48.5 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

EN1 Protein is essential for the correct formation of the apical ectodermal ridge and accurate dorsal-ventral patterning in the limb. EN1 Protein, Gallus gallus (His-B2M) is the recombinant EN1 protein, expressed by E. coli , with N-6*His, B2M labeled tag.
研究背景

EN1 在胚胎发育中发挥着关键作用，特别有助于顶端外胚层脊的正确形成，并确保肢体发育中准确的背腹模式。
种属

Others
表达系统

E. coli
标签

N-6*His;B2M
蛋白编号

Q05916 (M1-E333)
基因 ID

771008  [NCBI]
蛋白结构
N-term
6*His-B2M
EN1 (M1-333E)
Accession # Q05916
C-term
同用名
EN1; EN-1; Homeobox protein engrailed-1; Gg-En-1; Homeobox protein en-1
氨基酸序列

MEEPPEGHGHHRDAAPPGPANGGGGGGGGSDGDSAPVSPSPAPASPAAPCPLPLPRRRPPPPPPPRTTNFFIDNILRPDFGCKKEPPAATGAATGAGGGGGGGGREQRDGAQSAGRENVNPLLARPPHAPSSALLCPDSNCAPDGSAPAGTAAKANPGTAAGAAGAAGAAKAQGDGGETPAAKYGEHGSPAILLMGSNNGGAVLKPDSQQPLVWPAWVYCTRYSDRPSSPRTRKLKKKKTEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQSLAQELSLNESRVKIWFQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKEESE

分子量

Approximately 48.5 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

EN1 Protein, Gallus gallus (His-B2M) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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