1. 重组蛋白
  2. Enzymes & Regulators
  3. Lyases (EC 4)
  4. Enolase 1/ENO1 蛋白, Rat (His)

Enolase 1/ENO1 蛋白, Rat (His)

目录号: HY-P75253
COA 产品使用指南

烯醇化酶 1 (ENO1) 在碳水化合物降解中发挥着关键作用,特别是在糖酵解途径中,它催化 2-磷酸-D-甘油酸转化为磷酸烯醇丙酮酸。这种酶活性发生在糖酵解的第四步中,标志着葡萄糖转化为丙酮酸的关键阶段。Enolase 1/ENO1 蛋白, Rat (His) 是重组的 烯醇化酶 ENO1 蛋白,由 E. coli 表达,带有 N-His 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥720 In-stock
10 μg ¥1200 In-stock
50 μg ¥3400 In-stock
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Enolase 1 (ENO1) plays a key role in carbohydrate degradation, particularly in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate.This enzyme activity occurs during the fourth step of glycolysis and marks a critical stage in the conversion of glucose to pyruvate.Enolase 1/ENO1 Protein, Rat (His) is the recombinant rat-derived Enolase 1/ENO1 protein, expressed by E.coli , with N-His labeled tag.

研究背景

烯醇化酶 1 (ENO1) 是参与碳水化合物降解的关键酶,特别是在糖酵解途径中,它催化 2-磷酸甘油酸转化为磷酸烯醇丙酮酸。该反应发生在糖酵解的倒数第二步中,对于从 D-甘油醛 3-磷酸生成丙酮酸至关重要。ENO1通过参与糖酵解过程,将中间体转化为丙酮酸,从而进一步进入各种代谢途径,在细胞能量代谢中发挥核心作用。ENO1 的酶活性有助于 ATP 的产生,使其成为细胞能量产生的重要组成部分。多项研究表明ENO1参与糖酵解过程中从D-甘油醛3-磷酸到丙酮酸的特定生化转化,强调了其在细胞能量代谢中的重要性。

生物活性

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

种属

Rat

表达系统

E. coli

标签

N-His

蛋白编号

Q5BJ93 (M1-K434)

基因 ID
蛋白结构
N-term
His
ENO1 (M1-K434)
Accession # Q5BJ93
C-term
同用名
ENO1L1; Eno-1; Alpha-enolase; NNE; MBP-1; Enolase 1
分子量

Approximately 49.4 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 10% Glycerol, pH 8.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Enolase 1/ENO1 Protein, Rat (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
Enolase 1/ENO1 Protein, Rat (His)
目录号:
HY-P75253
需求量: