Enoyl-ACP Reductase 蛋白, E. coli (His)

Enoyl-ACP Reductase Protein is a member of the Short chain Dehydrogenase Reductase (SDR) superfamily.It is a key enzyme of the type II fatty acid synthesis (FAS) system.Enoyl-ACP Reductase catalyzes the reduction of a carbon-carbon double bond in an enoyl moiety that is covalently linked to an acyl carrier protein (ACP).And it is involved in the elongation cycle of fatty acid which are used in the lipid metabolism and in the biotin biosynthesis.Enoyl-ACP Reductase Protein, E.coli (His) is the recombinant E.coli-derived Enoyl-ACP Reductase protein, expressed by E.coli , with N-His labeled tag.

烯酰-ACP 还原酶是短链脱氢酶还原酶 (SDR) 超家族的成员，因此在结构和机制上与脂肪酸合成循环的其他 SDR 酶 3-酮脂酰-ACP 还原酶密切相关。烯酰-ACP 还原酶是 II 型脂肪酸合成 (FAS) 系统的关键酶。脂肪酸生物合成对于哺乳动物、植物、真菌和细菌 (古细菌产生类异戊二烯脂质) 的生存至关重要。烯酰基-ACP 还原酶催化与酰基载体蛋白 (ACP) 共价连接的烯酰基部分中的碳-碳双键还原。它参与脂质代谢和生物素生物合成中使用的脂肪酸的延伸循环^[1][2]。

Enzymatic activity is determined by following NADH consumption. The specific activity is 1055.75 pmol/min/μg.

E.coli

E. coli

N-6*His

WP_000506490 (G2-K262)

/

GFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKLKGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDGDYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMGLAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRRTVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK

Approximately 30 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.