1. 重组蛋白
  2. Enzymes & Regulators Biotinylated Proteins
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-3
  5. ENPP-3 蛋白, Human (828a.a, HEK293, His-Avi)

ENPP-3 蛋白, Human (828a.a, HEK293, His-Avi)

目录号: HY-P77924
产品使用指南

ENPP3是一种水解酶,可代谢细胞外核苷酸,包括ATP、GTP、UTP和CTP,在免疫调节中发挥关键作用。它通过消除细胞外 ATP(炎症细胞因子释放的触发因素)来调节炎症和慢性过敏期肥大细胞和嗜碱性粒细胞的反应。ENPP-3 蛋白, Human (828a.a, HEK293, His-Avi) 是重组的 ENPP-3 蛋白,由 HEK293 表达,带有 N-His, N-Avi 标签。ENPP-3 蛋白, Human (828a.a, HEK293, His-Avi) 全长 828 个氨基酸,分子量为 110-120 kDa。

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描述

ENPP3 is a hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP, and CTP, and plays a key role in immune regulation. It modulates mast cell and basophil responses during inflammation and chronic allergic phases by eliminating extracellular ATP, a trigger for inflammatory cytokine release. ENPP-3 Protein, Human (828a.a, HEK293, His-Avi) is the recombinant human-derived ENPP-3 protein, expressed by HEK293 , with N-His, N-Avi labeled tag. The total length of ENPP-3 Protein, Human (828a.a, HEK293, His-Avi) is 828 a.a., with molecular weight of 110-120 kDa.

研究背景

ENPP3 是一种水解酶,在细胞外核苷酸(包括 ATP、GTP、UTP 和 CTP)代谢中发挥着关键作用。这种酶活性有助于调节免疫反应,特别是在炎症和慢性过敏阶段调节肥大细胞和嗜碱性粒细胞反应。ENPP3 通过消除细胞外 ATP(一种激活嗜碱性粒细胞和肥大细胞的信号分子,随后触发炎症细胞因子的释放)来实现这一目标。此外,在小肠腔内,ENPP3 代谢细胞外 ATP,有效防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外,ENPP3 还表现出碱性磷酸二酯酶活性,增加了其在细胞过程中的多种功能。

种属

Human

表达系统

HEK293

标签

N-His;N-Avi

蛋白编号

O14638 (L48-I875)

基因 ID
蛋白结构
N-term
His-Avi
ENPP-3 (L48-I875)
Accession # O14638
C-term
同用名
E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6
分子量

110-120 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ENPP-3 Protein, Human (828a.a, HEK293, His-Avi) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ENPP-3 Protein, Human (828a.a, HEK293, His-Avi)
目录号:
HY-P77924
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