1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-3 蛋白, Human (828a.a, HEK293, His)

ENPP-3 蛋白, Human (828a.a, HEK293, His)

目录号: HY-P700410
COA 产品使用指南

ENPP3是一种水解酶,可代谢细胞外核苷酸,包括ATP、GTP、UTP和CTP,在免疫调节中发挥关键作用。它通过消除细胞外 ATP(炎症细胞因子释放的触发因素)来调节炎症和慢性过敏期肥大细胞和嗜碱性粒细胞的反应。ENPP3 蛋白, Human (828a.a, HEK293, His) 是重组的 ENPP3 蛋白,由 HEK293 表达,带有 C-10*His 标签。

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规格 价格 是否有货 数量
10 μg ¥920 询价
20 μg ¥1470 In-stock
50 μg ¥2800 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

ENPP3 is a hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP, and CTP, and plays a key role in immune regulation. It modulates mast cell and basophil responses during inflammation and chronic allergic phases by eliminating extracellular ATP, a trigger for inflammatory cytokine release. ENPP3 Protein, Human (828a.a, HEK293, His) is the recombinant human-derived ENPP3 protein, expressed by HEK293 , with C-10*His labeled tag.

研究背景

ENPP3 是一种水解酶,在细胞外核苷酸(包括 ATP、GTP、UTP 和 CTP)代谢中发挥着关键作用。这种酶活性有助于调节免疫反应,特别是在炎症和慢性过敏阶段调节肥大细胞和嗜碱性粒细胞反应。ENPP3 通过消除细胞外 ATP(一种激活嗜碱性粒细胞和肥大细胞的信号分子,随后触发炎症细胞因子的释放)来实现这一目标。此外,在小肠腔内,ENPP3 代谢细胞外 ATP,有效防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外,ENPP3 还表现出碱性磷酸二酯酶活性,增加了其在细胞过程中的多种功能。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human ENPP3 at 2 μg/mL can bind anti-ENPP3 recombinant antibody, the EC50 is ≤ 2.868 ng/mL.

种属

Human

表达系统

HEK293

标签

C-10*His

蛋白编号

O14638 (L48-I875)

基因 ID
蛋白结构
N-term
ENPP3 (L48-I875)
Accession # O14638
10*His
C-term
同用名
E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6
氨基酸序列

LEKQGSCRKKCFDASFRGLENCRCDVACKDRGDCCWDFEDTCVESTRIWMCNKFRCGETRLEASLCSCSDDCLQRKDCCADYKSVCQGETSWLEENCDTAQQSQCPEGFDLPPVILFSMDGFRAEYLYTWDTLMPNINKLKTCGIHSKYMRAMYPTKTFPNHYTIVTGLYPESHGIIDNNMYDVNLNKNFSLSSKEQNNPAWWHGQPMWLTAMYQGLKAATYFWPGSEVAINGSFPSIYMPYNGSVPFEERISTLLKWLDLPKAERPRFYTMYFEEPDSSGHAGGPVSARVIKALQVVDHAFGMLMEGLKQRNLHNCVNIILLADHGMDQTYCNKMEYMTDYFPRINFFYMYEGPAPRIRAHNIPHDFFSFNSEEIVRNLSCRKPDQHFKPYLTPDLPKRLHYAKNVRIDKVHLFVDQQWLAVRSKSNTNCGGGNHGYNNEFRSMEAIFLAHGPSFKEKTEVEPFENIEVYNLMCDLLRIQPAPNNGTHGSLNHLLKVPFYEPSHAEEVSKFSVCGFANPLPTESLDCFCPHLQNSTQLEQVNQMLNLTQEEITATVKVNLPFGRPRVLQKNVDHCLLYHREYVSGFGKAMRMPMWSSYTVPQLGDTSPLPPTVPDCLRADVRVPPSESQKCSFYLADKNITHGFLYPPASNRTSDSQYDALITSNLVPMYEEFRKMWDYFHSVLLIKHATERNGVNVVSGPIFDYNYDGHFDAPDEITKHLANTDVPIPTHYFVVLTSCKNKSHTPENCPGWLDVLPFIIPHRPTNVESCPEGKPEALWVEERFTAHIARVRDVELLTGLDFYQDKVQPVSEILQLKTYLPTFETTI

分子量

Approximately 116 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

ENPP-3 Protein, Human (828a.a, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
ENPP-3 Protein, Human (828a.a, HEK293, His)
目录号:
HY-P700410
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