1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3) Phosphatase
  4. ENPP-3
  5. ENPP-3 蛋白, Rhesus Macaque (sf9, His)

ENPP-3 蛋白, Rhesus Macaque (sf9, His)

目录号: HY-P77357
产品使用指南

ENPP-3 是一种代谢细胞外核苷酸(ATP、GTP、UTP、CTP)的水解酶,对于炎症和慢性过敏反应的免疫调节至关重要。通过消除细胞外 ATP,ENPP-3 抑制嗜碱性粒细胞和肥大细胞活化并抑制炎症细胞因子的释放。ENPP-3 蛋白, Rhesus Macaque (sf9, His) 是重组的 ENPP-3 蛋白,由 Sf9 insect cells 表达,带有 N-His 标签。ENPP-3 蛋白, Rhesus Macaque (sf9, His) 全长 829 个氨基酸,分子量约为 ~97.2 KDa。

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描述

ENPP-3 is a hydrolase that metabolizes extracellular nucleotides (ATP, GTP, UTP, CTP), which is critical for immune regulation of inflammation and chronic allergic reactions. By eliminating extracellular ATP, ENPP-3 inhibits basophil and mast cell activation and suppresses the release of inflammatory cytokines. ENPP-3 Protein, Rhesus Macaque (sf9, His) is the recombinant Rhesus Macaque-derived ENPP-3 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of ENPP-3 Protein, Rhesus Macaque (sf9, His) is 829 a.a., with molecular weight of ~97.2 KDa.

研究背景

ENPP-3 是一种水解酶,具有代谢各种细胞外核苷酸的能力,包括 ATP、GTP、UTP 和 CTP。这种酶活性在调节免疫反应中发挥着至关重要的作用,特别是在炎症和过敏反应的慢性阶段。ENPP-3 通过消除细胞外 ATP 发挥作用,ATP 是一种激活嗜碱性粒细胞和肥大细胞的信号分子,导致炎症细胞因子的释放。此外,在小肠腔内,ENPP-3 代谢细胞外 ATP,从而防止 ATP 诱导的肠浆细胞样树突状细胞凋亡。除了参与核苷酸代谢外,ENPP-3 还表现出碱性磷酸二酯酶活性,增加了其在细胞过程中的多方面作用。

种属

Rhesus Macaque

表达系统

Sf9 insect cells

标签

N-His

蛋白编号

XP_001103528 (R46-I874)

基因 ID

710232  [NCBI]

蛋白结构
N-term
His
ENPP-3 (R46-I874)
Accession # XP_001103528
C-term
同用名
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3; NPP3; PD-Ibeta; PDNP3
分子量

Approximately 97.2 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

ENPP-3 Protein, Rhesus Macaque (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ENPP-3 Protein, Rhesus Macaque (sf9, His)
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