ENPP-7 蛋白, Mouse (HEK293, His)

The ENPP-7 protein acts as a choline-specific phosphodiesterase and is essential for sphingomyelin digestion, ceramide formation, and FA absorption in the gastrointestinal tract. It hydrolyzes sphingomyelin to release ceramide and phosphocholine. ENPP-7 Protein, Mouse (HEK293, His) is the recombinant mouse-derived ENPP-7 protein, expressed by HEK293 , with C-His labeled tag. The total length of ENPP-7 Protein, Mouse (HEK293, His) is 400 a.a., with molecular weight of ~60 kDa.

ENPP-7 蛋白作为胆碱特异性磷酸二酯酶发挥作用，在胃肠道内的鞘磷脂消化、神经酰胺形成和脂肪酸 (FA) 吸收中发挥着至关重要的作用。这种酶水解鞘磷脂，释放神经酰胺和磷酸胆碱。此外，ENPP-7 具有磷脂酶 C 活性，可将磷酸胆碱从棕榈酰溶血磷脂酰胆碱和血小板激活因子 (PAF) 中裂解出来，从而使 PAF 失活。尽管具有酶活性，ENPP-7 不具有核苷酸焦磷酸酶活性。该蛋白质参与这些过程表明，通过调节肠腔内饮食或内源性来源的鞘磷脂水平，在促进胆固醇吸收方面具有潜在作用。

Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit. In this experiment, 0.071 mM SPM substrate was used per well at 37°C for 30 min. The specific activity is 23492.16 pmol/min/μg.

Mouse

HEK293

C-10*His

Q3TIW9 (A22-Q421)

238011  [NCBI]

APVQRQHKLLLVSFDGFRWNYDQDVDTPNLDSMAQEGVKAQYMTPAFVTMTSPCHFTLVTGKYIENHGVVHNMFYNTTSTVRLPYHATLGIQRWWDNGSIPIWITAQRQGLKTGSFFYPGGNVTYQGEAVTMSRKEGVLHNYKNETEWRGNVDTVMKWFLEEDVSLVTLYFGEPDSTGHKYGPESQERKDMVKQVDRTVGYLRDSIKRHHLSDSLNLIITSDHGMTTVNKKASDLVEFHKFSNFTFQDIQFELLDYGPIGMLIPKEGMLEKVYSVLKDAHPRLHVYKKEDFPKNFHYANNPRITPLLMYSDLGYVIHGRVNVQFNNGEHGFNNQDMDMKTIFRAVGPSFKAGLEVEPFESVHVYELMCQLLGIVPEPNDGNPGILRPMLRSGSASLLSSQ

Approximately 57-70 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 150 mM NaCl, pH 7.6.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.