Enterokinase 蛋白, Human (HEK293, His)

Enterokinase Protein initiates the activation of pancreatic proteolytic proenzymes, including trypsin, chymotrypsin, and carboxypeptidase A. It catalyzes the conversion of trypsinogen to trypsin, triggering the activation of other proenzymes like chymotrypsinogen, procarboxypeptidases, and proelastases. Enterokinase Protein, Human (HEK293, His) is the recombinant human-derived Enterokinase protein, expressed by HEK293 , with C-10*His labeled tag. The total length of Enterokinase Protein, Human (HEK293, His) is 979 a.a., with molecular weight of 140-150 kDa.

肠激酶蛋白在胰腺蛋白水解酶原（包括胰蛋白酶、胰凝乳蛋白酶和羧肽酶 A）激活的启动中起着至关重要的作用。它催化胰蛋白酶原转化为胰蛋白酶，从而导致其他酶原（如胰凝乳蛋白酶原、羧肽酶原和原弹性蛋白酶）的激活。

Measured by its ability to cleave a colorimetric peptide substrate 100 μM Z-Lys-SBzl that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 24352.69 pmol/min/µg.

Human

HEK293

C-10*His

P98073 (L41-H1019)

5651

LTIKESQRGAALGQSHEARATFKITSGVTYNPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDENVKEELIQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNCPDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDILDIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFEDGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEPACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDIALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQKGKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKANFTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWHTACAENWTTQISNDVCQLLGLGSGNSSKPIFPTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHKSCGKKLAAQDITPKIVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAILGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNCSIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH

Approximately 140-150 kDa under reducing condition due to glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.