EphA1 蛋白, Mouse (HEK293, His)

The EphA1 protein is a receptor tyrosine kinase that promiscuously binds to ephrin-A ligands on neighboring cells, initiating contact-dependent bidirectional signaling. The forward signal originates from the receptor and the reverse signal originates from the ephrin ligand. EphA1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphA1 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA1 Protein, Mouse (HEK293, His) is 522 a.a., with molecular weight of ~68 kDa.

EphA1 蛋白是一种受体酪氨酸激酶，与相邻细胞上膜结合的肝配蛋白 A 家族配体混杂结合，启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导，而肝配蛋白配体下游的途径称为反向信号传导。EphA1 表现出与 EFNA3 和 EFNA4 的低亲和力结合，以及与 EFNA1 的高亲和力结合，这可能构成其同源/功能配体。被 EFNA1 激活后，EphA1 诱导细胞附着到细胞外基质，通过调节 ILK 和下游 RHOA 和 RAC 抑制细胞扩散和运动。此外，EphA1 在血管生成和调节细胞增殖中发挥作用。它还可能参与细胞凋亡过程，有助于其多样化的细胞功能。

Immobilized rmEphA1 at 2 µg/mL (100 µL/well) can bind Biotinylated rmEphrin-A1. The ED₅₀ for this effect is 28.67 ng/mL.

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  Immobilized rmEphA1 at 2 µg/mL (100 µL/well) can bind Biotinylated rmEphrin-A1. The ED₅₀ for this effect is 28.67 ng/mL.

Mouse

HEK293

C-His

Q60750 (E27-E548)

13835  [NCBI]

EEVTLMDTSTAQGELGWLLDPPETGWSEVQQMLNGTPLYMYQDCPIQEGGDTDHWLRSNWIYRGEEASRIYVELQFTVRDCKSFPGGAGPLGCKETFNLFYMESDQDVGIQLRRPLFQKVTTVAADQSFTIRDLASGSVKLNVERCSLGHLTRRGLYLAFHNPGSCVALVSVRVFYQRCAETVHGLAHFPDTLPGPGGLVEVAGTCLSHAQISLGSSGTPRMHCSPDGEWLVPVGQCQCEPGYEESSGNVGCTACPTGFYRVDMNTLRCLKCPQHSIAESEGSTICTCENGHYRAPGEGPQVACTRPPSAPQNLSFSTSGTQLSLRWEPPRDTGGRHDIRYSVECLQCRGIAQDGGPCQPCGKGVHFSPAASGLTTSTVQVQGLEPYANYTFTVKSQNRVSGLDSSSPSSASLSINMGHAESLSGLSLKLVKKEPRQLELTWAGSRPRNPGGNLSYELHVLNQDEEWHQMVLEPRVLLTKLQPDTTYIVRVRTLTPLGPGPFSPDHEFRTSPPVSRSLTGGE

Approximately 68 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.