EphA2 蛋白, Human (HEK293, His)

EphA2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with various ephrin A ligands, affecting cell migration, adhesion, proliferation and differentiation. It regulates cell adhesion and differentiation by interacting with DSG1 and inhibiting the ERK1/ERK2 signaling pathway. EphA2 Protein, Human (HEK293, His) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA2 Protein, Human (HEK293, His) is 511 a.a., with molecular weight of 65-75 kDa.

EphA2 蛋白是一种受体酪氨酸激酶，可与邻近细胞上的各种膜结合肝配蛋白 A 家族配体相互作用，从而产生双向信号传导。该受体通过正向和反向信号传导途径参与调节细胞迁移、粘附、增殖和分化。它通过与 DSG1/desmoglein-1 相互作用并抑制 ERK1/ERK2 信号通路在细胞粘附和分化中发挥作用。此外，EphA2 蛋白可能参与紫外线辐射诱导的细胞凋亡，并独立于配体结合刺激趋化细胞迁移。在发育过程中，它有助于胎儿组织的模式形成和发育，包括血管生成、后脑发育和乳腺形态发生。EphA2 蛋白还与 Ephrin-A5/EFNA5 相互作用，调节晶状体纤维细胞的形状和相互作用，这对于维持晶状体透明度至关重要。此外，它通过与 Ephrin-A2/EFNA2 相互作用调节破骨细胞生成和成骨细胞生成，从而在骨重塑中发挥作用。值得注意的是，EphA2 蛋白在肝细胞中充当丙型肝炎病毒 (HCV) 的受体，通过促进 CD81-CLDN1 受体复合物的形成并增强与 HCV 包膜糖蛋白的膜融合来促进病毒细胞进入。

1. Measured by its binding ability in a functional ELISA. Immobilized rhEphA2 at 2 µg/mL (100 µL/well) can bind rmEphrin-A1. The ED₅₀ for this effect is 4.594-4.847 ng/mL.
2. Measured in a cell proliferation assay using PC-3 cells. The ED₅₀ for this effect is 0.8703 ng/mL, corresponding to a specific activity is 1.15×10⁶ units/mg.

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  Measured in a cell proliferation assay using PC-3 cells.The ED₅₀ for this effect is 0.8703 ng/mL, corresponding to a specific activity is 1.15×10⁶ units/mg.

Human

HEK293

C-His

P29317/NP_004422.2 (A24-N534)

1969  [NCBI]

AQGKEVVLLDFAAAGGELGWLTHPYGKGWDLMQNIMNDMPIYMYSVCNVMSGDQDNWLRTNWVYRGEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDLDYGTNFQKRLFTKIDTIAPDEITVSSDFEARHVKLNVEERSVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPELLQGLAHFPETIAGSDAPSLATVAGTCVDHAVVPPGGEEPRMHCAVDGEWLVPIGQCLCQAGYEKVEDACQACSPGFFKFEASESPCLECPEHTLPSPEGATSCECEEGFFRAPQDPASMPCTRPPSAPHYLTAVGMGAKVELRWTPPQDSGGREDIVYSVTCEQCWPESGECGPCEASVRYSEPPHGLTRTSVTVSDLEPHMNYTFTVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEGRSTTSLSVSWSIPPPQQSRVWKYEVTYRKKGDSNSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSPEGSGN

Approximately 65-75 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 5% Trehalose, pH7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.