EphA2 蛋白, Human (Biotinylated, HEK293, His-Avi)

The EphA2 protein is an important member of the ephrin receptor subfamily that mediates developmental events, particularly in the nervous system. It has a single kinase domain and an extracellular region that binds ephrin-A ligands. EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived EphA2 protein, expressed by HEK293 , with C-6*His, C-Avi labeled tag. The total length of EphA2 Protein, Human (Biotinylated, HEK293, His-Avi) is 511 a.a., with molecular weight of ~59.4 kDa.

EphA2 蛋白是蛋白酪氨酸激酶家族中肝配蛋白受体亚家族的成员，在介导发育事件中发挥着至关重要的作用，特别是在神经系统中。EPH 受体具有单个激酶结构域和具有富含 Cys 结构域和 2 个纤连蛋白 III 型重复的胞外区域，参与结合肝配蛋白 A 和肝配蛋白 B 配体。EphA2 特异性结合肝配蛋白-A 配体，并在多种组织中广泛表达，包括食道 (RPKM 37.8)、膀胱 (RPKM 18.3) 和 21 个其他组织。此外，该基因的突变与某些遗传相关的白内障疾病有关，突出了其在发育过程和眼部健康中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized EFNA1-hFc at 10 μg/mL (100 μL/well) can bind Biotinylated EphA2 Protein, Human (HEK293, His-Avi) and the EC₅₀ is 20-40 ng/mL.

Human

HEK293

C-6*His;C-Avi

NP_004422.2 (A24-N534)

1969  [NCBI]

LKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI

Approximately 59.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.