EphA2 蛋白, Human (sf9, His-GST)

EphA2 protein is a receptor tyrosine kinase that participates in bidirectional signaling with various ephrin A ligands, affecting cell migration, adhesion, proliferation and differentiation. It regulates cell adhesion and differentiation by interacting with DSG1 and inhibiting the ERK1/ERK2 signaling pathway. EphA2 Protein, Human (sf9, His-GST) is the recombinant human-derived EphA2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of EphA2 Protein, Human (sf9, His-GST) is 392 a.a., with molecular weight of ~62 kDa.

EphA2 蛋白是一种受体酪氨酸激酶，可与邻近细胞上的各种膜结合肝配蛋白 A 家族配体相互作用，从而产生双向信号传导。该受体通过正向和反向信号传导途径参与调节细胞迁移、粘附、增殖和分化。它通过与 DSG1/desmoglein-1 相互作用并抑制 ERK1/ERK2 信号通路在细胞粘附和分化中发挥作用。此外，EphA2 蛋白可能参与紫外线辐射诱导的细胞凋亡，并独立于配体结合刺激趋化细胞迁移。在发育过程中，它有助于胎儿组织的模式形成和发育，包括血管生成、后脑发育和乳腺形态发生。EphA2 蛋白还与 Ephrin-A5/EFNA5 相互作用，调节晶状体纤维细胞的形状和相互作用，这对于维持晶状体透明度至关重要。此外，它通过与 Ephrin-A2/EFNA2 相互作用调节破骨细胞生成和成骨细胞生成，从而在骨重塑中发挥作用。值得注意的是，EphA2 蛋白在肝细胞中充当丙型肝炎病毒 (HCV) 的受体，通过促进 CD81-CLDN1 受体复合物的形成并增强与 HCV 包膜糖蛋白的膜融合来促进病毒细胞进入。

The specific activity was determined to be 50 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Human

Sf9 insect cells

N-His;N-GST

P29317 (L585-I976)

1969  [NCBI]

LKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI

Approximately 62 kDa

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 3 mM DTT, pH 8.5, 10% gly

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.