EphA2 蛋白, Mouse (HEK293, His)

EphA2 protein is a receptor tyrosine kinase that promiscuously binds to ephrin A ligands to initiate bidirectional signaling. EphA2 is activated by ephrin-A1/EFNA1, regulates cell migration, adhesion, proliferation and differentiation, modulates DSG1 and inhibits ERK1/ERK2 signaling. EphA2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived EphA2 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphA2 Protein, Mouse (HEK293, His) is 510 a.a., with molecular weight of ~65-95 kDa.

EphA2 蛋白是一种受体酪氨酸激酶，与相邻细胞上膜结合的肝配蛋白 A 家族配体混杂结合，启动接触依赖性双向信号传导。源自受体的下游途径称为正向信号传导，而肝配蛋白配体下游的途径称为反向信号传导。EphA2 由配体肝配蛋白-A1/EFNA1 激活，在细胞迁移、整合素介导的粘附、增殖和分化中发挥调节作用。此外，EphA2 通过 DSG1/desmoglein-1 调节细胞粘附和分化，并抑制 ERK1/ERK2 信号通路。它还可能参与紫外线辐射诱导的细胞凋亡，并对趋化细胞迁移表现出配体独立的刺激作用。在发育过程中，EphA2 在模式形成的各个方面发挥作用，并有助于多种胎儿组织的发育，包括血管生成、早期后脑发育以及乳腺发育过程中的上皮增殖和分支形态发生。与配体 ephrin-A5/EFNA5 的相互作用可以调节晶状体纤维细胞的形状和相互作用，在晶状体透明度的发育和维持中发挥至关重要的作用。此外，EphA2 与 ephrin-A2/EFNA2 合作，可能通过调节破骨细胞生成和成骨细胞生成来促进骨重塑。

Measured by its binding ability in a functional ELISA. Immobilized mouse EphA2 at 2 μg/mL (100 μl/well) can bind mouse EphrinA1 with a linear range of 0.16-20 ng/mL.

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  Immobilized Recombinant Mouse EphA2 Proteinat 2 μg/mL (100 μL/well) can bind Recombinant Mouse Ephrin-A1 / EFNA1 Protein, the ED₅₀ is 11.4 ng/mL.

Mouse

HEK293

C-His

Q03145 (K26-N535)

13836  [NCBI]

KEVVLLDFAAMKGELGWLTHPYGKGWDLMQNIMDDMPIYMYSVCNVVSGDQDNWLRTNWVYREEAERIFIELKFTVRDCNSFPGGASSCKETFNLYYAESDVDYGTNFQKRQFTKIDTIAPDEITVSSDFEARNVKLNVEERMVGPLTRKGFYLAFQDIGACVALLSVRVYYKKCPEMLQSLARFPETIAVAVSDTQPLATVAGTCVDHAVVPYGGEGPLMHCTVDGEWLVPIGQCLCQEGYEKVEDACRACSPGFFKSEASESPCLECPEHTLPSTEGATSCQCEEGYFRAPEDPLSMSCTRPPSAPNYLTAIGMGAKVELRWTAPKDTGGRQDIVYSVTCEQCWPESGECGPCEASVRYSEPPHALTRTSVTVSDLEPHMNYTFAVEARNGVSGLVTSRSFRTASVSINQTEPPKVRLEDRSTTSLSVTWSIPVSQQSRVWKYEVTYRKKGDANSYNVRRTEGFSVTLDDLAPDTTYLVQVQALTQEGQGAGSKVHEFQTLSTEGSAN

Approximately 65-95 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 5%Trehalose, 5% Mannitol, 0.01%Tween-80, pH 7.4 or PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.