EphA4 蛋白, Human (sf9, His-GST)

The EphA4 protein is a receptor tyrosine kinase that participates in bidirectional signaling with membrane-bound ephrin ligands including EFNA1 and EFNB3. EphA4 is activated by GPI-anchored ephrin A and transmembrane ephrin B and regulates cell morphology and integrin-dependent adhesion through regulation of Rac, Rap, and Rho GTPases. EphA4 Protein, Human (sf9, His-GST) is the recombinant human-derived EphA4 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

EphA4 蛋白是一种受体酪氨酸激酶，可与相邻细胞上膜结合的肝配蛋白配体相互作用，从而实现双向信号传导。它可以被 GPI 锚定的肝配蛋白-A 和跨膜肝配蛋白-B 配体（例如 EFNA1 和 EFNB3）激活。EphA4 的激活通过调节 Rac、Rap 和 Rho GTPases 的活性来调节细胞形态和整合素依赖性细胞粘附。在神经系统中，EphA4 在轴突引导中发挥着至关重要的作用，包括神经肌肉回路发育过程中的皮质脊髓投射以及运动和感觉轴突的分离。它还有助于突触可塑性，并参与损伤后的修复，防止轴突再生。EphA4 的混杂性使其能够参与各种细胞间信号传导过程，包括胸腺上皮的发育。此外，它通过与 ADAM10 和 CADH1 形成复合物来调节 Corti 耳蜗器官中的柱细胞分离，从而导致粘附连接的破坏。EphA4 磷酸化 CAPRIN1，促进无膜区室的形成。

Human

Sf9 insect cells

N-His;N-GST

P54764-1 (S570-V986)

2043  [NCBI]

SRRRSKYSKAKQEADEEKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV

Approximately 67 kDa

Greater than 90% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.