EphB1 蛋白, Rhesus Macaque (HEK293, His)

EphB1, a tyrosine kinase receptor, was promiscuously bound to the transmembrane ligand ephrin-B family on adjacent cells, initiating contact-dependent bidirectional signaling. EphB1 activates the MAPK/ERK and JNK signaling cascades that regulate cell migration and adhesion, respectively. EphB1 promotes differentiation and maturation of dendritic cells in non-small cell lung cancer. EphB1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived EphB1 protein, expressed by HEK293 , with C-His labeled tag.

EphB1 蛋白是一种酪氨酸激酶受体，与邻近细胞上的跨膜 ephrin-B 家族配体混杂结合，启动接触依赖的双向信号传导。源自受体的下游信号通路称为正向信号通路，而 ephrin 配体的下游信号通路称为反向信号通路。该受体的同源/功能性 ephrin 配体包括 EFNB1、EFNB2 和 EFNB3。在神经系统发育中，EphB1 通过重定向视交叉中线的同侧腹颞视网膜神经节细胞轴突来调节视网膜轴突的引导，可能是通过与 EFNB2的排斥相互作用。在成人神经系统中，EphB1 与 EFNB3 一起控制海马神经祖细胞的趋化性、增殖和极性。除了轴突引导外，EphB1 还与其他 ephrin-B 受体在树突棘的发育和成熟以及突触的形成中起着至关重要的冗余作用。EphB1 可能调节血管生成，在靶向细胞迁移和粘附中发挥作用。在被 EFNB1 和其他可能的 ephrin-B 配体激活后，EphB1 激活 MAPK/ERK 和 JNK 信号级联，分别调节细胞迁移和粘附。EphB1 通过促进卫星细胞 (肌肉干细胞) 的自我更新和减少其激活和分化，参与维持卫星细胞池 (肌肉干细胞)。EphB1 促进非小细胞肺癌树突状细胞的分化和成熟。

Measured by its binding ability in a functional ELISA. Immobilized Human EFNB1 at 2 μg/ml (100 μl/well) can bind Rhesus Macaque EPHB1. The ED₅₀ for this effect is 14.49 ng/mL.

• 
  Measured by its binding ability in a functional ELISA. Immobilized Human EFNB1 at 2 μg/ml (100 μL/well) can bind Rhesus Macaque EPHB1. The ED₅₀ for this effect is 14.49 ng/mL.

Rhesus Macaque

HEK293

C-10*His

XP_001115263.1 (M18-P540)

717211  [NCBI]

MEETLMDTRTATAELGWTANPASGWEEVSGYDENLNTIRTYQVCNVFEPNQNNWLLTTFINRRGAHRIYTEMRFTVRDCSSLPNVPGSCKETFNLYYYETDSVIATKKSAFWSEAPYLKVDTIAADESFSQVDFGGRLMKVNTEVRSFGPLTRNGFYLAFQDYGACMSLLSVRVFFKKCPSIVQNFAVFPETMTGAESTSLVIARGTCIPNAEEVDVPIKLYCNGDGEWMVPIGRCTCKPGYEPENSVACKACPAGTFKASQEAEGCSHCPSNSRSPAEASPICTCRTGYYRADFDPPEVACTSVPSGPRNVISIVNETSIILEWHPPRETGGRDDVTYNIICKKCRADRRSCSRCDDNVEFVPRQLGLTECRVSISSLWAHTPYTFDIQAINGVSSKSPFPPQHVSVNITTNQAAPSTVPIMHQVSATMRSITLSWPQPEQPNGIILDYEIRYYEKEHNEFNSSMARSQTNTARIDGLRPGMVYVVQVRARTVAGYGKFSGKMCFQTLTDDDYKSELREQLP

Approximately 58-77 kDa due to the glycosylation.

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.