1. 重组蛋白
  2. Cytokines and Growth Factors Receptor Proteins Enzymes & Regulators
  3. Ephrin/Eph Family Receptor Tyrosine Kinases Protein Tyrosine Kinases
  4. Eph Receptors
  5. EphB3
  6. EphB3 蛋白, Human (HEK293, His)

EphB3 蛋白, Human (HEK293, His)

目录号: HY-P77931
产品使用指南

EphB3 蛋白是一种受体酪氨酸激酶,与跨膜肝配蛋白 B 配体执行双向信号传导。它参与前向信令和反向信令,与 EPHB2 共享功能。EphB3 蛋白, Human (HEK293, His) 是重组的 EphB3 蛋白,由 HEK293 表达,带有 C-His 标签。EphB3 蛋白, Human (HEK293, His) 全长 526 个氨基酸,分子量为 65-68 kDa。

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描述

The EphB3 protein is a receptor tyrosine kinase that performs bidirectional signaling with the transmembrane ephrin B ligand. It participates in forward signaling and reverse signaling, sharing functionality with EPHB2. EphB3 Protein, Human (HEK293, His) is the recombinant human-derived EphB3 protein, expressed by HEK293 , with C-His labeled tag. The total length of EphB3 Protein, Human (HEK293, His) is 526 a.a., with molecular weight of 65-68 kDa.

研究背景

EphB3 蛋白是一种受体酪氨酸激酶,通过与跨膜肝配蛋白-B 家族配体结合,参与与相邻细胞的接触依赖性双向信号传导。该受体参与受体下游的正向信号传导和肝配蛋白配体下游的反向信号传导。EphB3EPHB2 具有重叠和冗余的功能,特别是在发育过程中的轴突引导方面,它调节大脑皮层中半球间连接的形成。此外,EphB3 有助于树突棘和兴奋性突触的发育和成熟,并控制细胞在各种发育过程中的迁移和定位,如血管生成、腭发育和胸腺上皮发育。EFNB2/EPHB3 复合物还在调节参与尿道管状化和泄殖腔分隔的细胞的迁移和粘附中发挥作用。最后,EphB3 对于肠上皮分化至关重要,可区分隐窝内的祖细胞和分化细胞。

种属

Human

表达系统

HEK293

标签

C-His

蛋白编号

P54753 (G34-L559)

基因 ID
蛋白结构
N-term
EphB3 (G34-L559)
Accession # P54753
His
C-term
同用名
EK2; ETK2; HEK2; TYRO6; EPHB3; AW456895; Cek10; MDK5; Sek4; EC 2.7.10; EC 2.7.10.1
分子量

65-68 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EphB3 Protein, Human (HEK293, His)
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