2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Ephrin/Eph Family
  5. Ephrins
  6. Ephrin-A4
  7. Ephrin-A4/EFNA4 蛋白, Human (HEK293, His)

Ephrin-A4/EFNA4 蛋白, Human (HEK293, His)

目录号: HY-P70376
产品使用指南

Ephrin-A4/EFNA4 蛋白是一种 GPI 结合配体，与 Eph 受体相互作用，对神经元、血管和上皮发育至关重要。它通过与邻近的 Eph 受体结合，启动双向信号传导，实现迁移、排斥和粘附。此外，它还可能促进扁桃体中活化的 B 淋巴细胞和树突状细胞之间的相互作用。Ephrin-A4/EFNA4 蛋白, Human (HEK293, His) 是重组的 Ephrin-A4/EFNA4 蛋白，由 HEK293 表达，带有 C-6*His 标签。Ephrin-A4/EFNA4 蛋白, Human (HEK293, His) 全长 146 个氨基酸，分子量约为 ~20.0 kDa。

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描述

Ephrin-A4/EFNA4 Protein, a GPI-bound ligand, interacts with Eph receptors, crucial for neuronal, vascular, and epithelial development. It enables migration, repulsion, and adhesion by binding to neighboring Eph receptors, initiating bidirectional signaling. Furthermore, it potentially facilitates the interaction between activated B-lymphocytes and dendritic cells in tonsils. Ephrin-A4/EFNA4 Protein, Human (HEK293, His) is the recombinant human-derived Ephrin-A4/EFNA4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Ephrin-A4/EFNA4 Protein, Human (HEK293, His) is 146 a.a., with molecular weight of ~20.0 kDa.
研究背景

Ephrin-A4/EFNA4 蛋白是一种细胞表面 GPI 结合配体，可与 Eph 受体（对神经元、血管和上皮发育至关重要的受体酪氨酸激酶家族）相互作用，从而实现迁移、排斥和粘附。它与邻近细胞上的 Eph 受体混杂结合，启动接触依赖性双向信号传导。此外，Ephrin-A4/EFNA4 蛋白可能有助于扁桃体中活化的 B 淋巴细胞和树突状细胞之间的相互作用。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

P52798 (L26-G171)
基因 ID
蛋白结构
N-term
EFNA4 (L26-G171)
Accession # P52798
6*His
C-term
同用名
rHuEphrin-A4/EFNA4, His; Ephrin-A4; EPH-Related Receptor Tyrosine Kinase Ligand 4; LERK-4; EFNA4; EPLG4; LERK4
氨基酸序列

LRHVVYWNSSNPRLLRGDAVVELGLNDYLDIVCPHYEGPGPPEGPETFALYMVDWPGYESCQAEGPRAYKRWVCSLPFGHVQFSEKIQRFTPFSLGFEFLPGETYYYISVPTPESSGQCLRLQVSVCCKERKSESAHPVGSPGESG

分子量

Approximately 20.0 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ephrin-A4/EFNA4 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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