2. 重组蛋白
  3. Cytokines and Growth Factors
  4. Ephrin/Eph Family
  5. Ephrins
  6. Ephrin-B1
  7. Ephrin-B1/EFNB1 蛋白, Mouse (HEK293, His)

Ephrin-B1/EFNB1 蛋白, Mouse (HEK293, His)

目录号: HY-P73028
产品使用指南

Ephrin-B1/EFNB1 蛋白是 Eph 受体的细胞表面配体，在神经元、血管和上皮发育过程中关键调节迁移、排斥和粘附。Ephrin-B1/EFNB1 蛋白, Mouse (HEK293, His) 是重组的 Ephrin-B1/EFNB1 蛋白，由 HEK293 表达，带有 C-His 标签。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

Ephrin-B1/EFNB1 proteins are cell surface ligands of Eph receptors that critically regulate migration, repulsion, and adhesion during neuronal, vascular, and epithelial development. Ephrin-B1/EFNB1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Ephrin-B1/EFNB1 protein, expressed by HEK293 , with C-His labeled tag.
研究背景

Ephrin-B1/EFNB1 是 Eph 受体的细胞表面跨膜配体，在神经元、血管和上皮发育过程中介导接触依赖性双向信号传导中发挥着关键作用。它与受体酪氨酸激酶 EPHB1/ELK 具有高亲和力，还与 EPHB2 和 EPHB3 结合。与邻近细胞上的 Eph 受体结合启动对迁移、排斥和粘附至关重要的双向信号传导。此外，EFNB1 参与体外诱导连合轴突/生长锥的塌陷，并可能有助于限制纵向突出轴突的方向。该蛋白通过其 PDZ 结合基序与 GRIP1 和 GRIP2 相互作用，并与 TLE1 结合。此外，EFNB1的胞内结构域肽与ZHX2相互作用，增强ZHX2的转录抑制活性。
种属

Mouse
表达系统

HEK293
标签

C-His
蛋白编号

P52795/NP_034240.1 (K30-S229)
基因 ID
蛋白结构
N-term
EFNB1 (K30-S229)
Accession # P52795/NP_034240.1
His
C-term
同用名
Ephrin-B1; EFL-3; ELK-L; LERK-2; Ephrin-B1 CTF; EFNB1; EFL3; EPLG2; LERK2
氨基酸序列

MARPGQRWLSKWLVAMVVLTLCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNKPHQEIRFTIKFQEFSPNYMGLEFKKYHDYYITSTSNGSLEGLENREGGVCRTRTMKIVMKVGQDPNAVTPEQLTTSRPSKESDNTVKTATQAPGRGSQGDSDGKHETVNQEEKSGPGAGGGGSGDS

分子量

33-38 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ephrin-B1/EFNB1 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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