1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Ephrin/Eph Family
  4. Ephrins
  5. Ephrin-B1
  6. Ephrin-B1/EFNB1 蛋白, Rat (HEK293, Fc)

Ephrin-B1/EFNB1 蛋白, Rat (HEK293, Fc)

目录号: HY-P73029
产品使用指南

Ephrin-B1/EFNB1 蛋白与邻近细胞上的 Eph 受体结合,促进发育中的神经元、血管和上皮细胞的双向信号传导。它对 EPHB1/ELK 具有高亲和力,还结合 EPHB2EPHB3,并诱导连合轴突/生长锥塌陷。Ephrin-B1/EFNB1 蛋白, Rat (HEK293, Fc) 是重组的 Ephrin-B1/EFNB1 蛋白,由 HEK293 表达,带有 C-hFc 标签。

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描述

Ephrin-B1/EFNB1 proteins bind to Eph receptors on neighboring cells and promote bidirectional signaling in developing neurons, blood vessels, and epithelia.It has high affinity for EPHB1/ELK, also binds EPHB2 and EPHB3, and induces commissural axon/growth cone collapse.Ephrin-B1/EFNB1 Protein, Rat (HEK293, Fc) is the recombinant rat-derived Ephrin-B1/EFNB1 protein, expressed by HEK293 , with C-hFc labeled tag.

研究背景

Ephrin-B1/EFNB1 蛋白是 Eph 受体的细胞表面跨膜配体,对神经元、血管和上皮发育至关重要,通过与相邻细胞上的 Eph 受体结合来参与接触依赖性双向信号传导。EFNB1 与受体酪氨酸激酶 EPHB1/ELK 具有高亲和力,因此还可以结合 EPHB2EPHB3。在体外,EFNB1 与连合轴突/生长锥结合,诱导其塌陷,并可能在限制纵向突出轴突的方向方面发挥作用。该蛋白的相互作用延伸至通过其 PDZ 结合基序与 GRIP1 和 GRIP2 结合,并与 TLE1 相互作用。此外,EFNB1的胞内结构域肽与ZHX2相互作用,增强ZHX2的转录抑制活性。这些多方面的相互作用强调了 EFNB1 在协调复杂的信号传导事件中的作用,有助于各种发育过程和细胞功能。

种属

Rat

表达系统

HEK293

标签

C-hFc

蛋白编号

P52796 (A25-T229)

基因 ID
蛋白结构
N-term
EFNB1 (A25-T229)
Accession # P52796
hFc
C-term
同用名
Ephrin-B1; EFL-3; ELK-L; LERK-2; Ephrin-B1 CTF; EFNB1; EFL3; EPLG2; LERK2
氨基酸序列

MARPGQRWLSKWLVAMVVLTLCRLATPLAKNLEPVSWSSLNPKFLSGKGLVIYPKIGDKLDIICPRAEAGRPYEYYKLYLVRPEQAAACSTVLDPNVLVTCNKPQQEIRFTIKFQEFSPNYMGLEFKKYHDYYITSTSNGSLEGLENREGGVCRTRTMKIVMKVGQDPNAVTPEQLTTSRPSKESDNTVKTATQAPGRGSQGDSDGKHETVNQQEKSGPGAGGSGSGDT

分子量

Approximately 58 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Ephrin-B1/EFNB1 Protein, Rat (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Ephrin-B1/EFNB1 Protein, Rat (HEK293, Fc)
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HY-P73029
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